Page 497 - IJB-10-2

International

Journal of Bioprinting

RESEARCH ARTICLE
Biomimetic mineralization of 3D-printed

polyhydroxyalkanoate-based microbial scaffolds
for bone tissue engineering

Dahong Kim , Su Jeong Lee , Dongjin Lee , Ji Min Seok , Seon Ju Yeo ,
1
1,2
1
1,2
3
Hyungjun Lim , Jae Jong Lee , Jae Hwang Song , Kangwon Lee , Won Ho Park ,
1
1
4
6
2,5
and Su A Park *
1
1 Nano-Convergence Manufacturing Systems Research Division, Korea Institute of Machinery and
Materials (KIMM), Daejeon, Republic of Korea
2 Department of Applied Bioengineering, Graduate School of Convergence Science and Technology,
Seoul National University, Seoul, Republic of Korea
3
Department of Microbiology, CHA University, Seongnam, Republic of Korea
4 Department of Orthopaedic Surgery, Konyang University Hospital, Daejeon, Republic of Korea
5 Research Institute for Convergence Science, Seoul National University, Seoul, Republic of Korea
6 Department of Organic Materials Engineering, Chungnam National University, Daejeon, Republic
of Korea
(This article belongs to the Special Issue: Advancements in 3D Printing, Microfluidics, and Their Integrated
Applications)
Abstract
Polyhydroxyalkanoates (PHAs) have gained much attention as a potential alternative to
conventional plastic bone scaffolds due to their biocompatibility and biodegradability,
among a diverse range of advantageous properties. However, the water resistance of
* Corresponding author: PHA creates an environment that can interfere with cell interactions. In this study, a three-
Su A Park dimensional-printed PHA scaffold was fabricated through fused deposition modeling
(psa@kimm.re.kr)
printing considering the physical properties of PHA. The PHA bone scaffolds were then
Citation: Kim D, Lee SJ, Lee D, coated with polydopamine (pDA) and/or hydroxyapatite (HA) in various configurations
et al. Biomimetic mineralization using a relatively simple and rapid process involving only immersion. The PHA–pDA–
of 3D-printed polyhydroxyal-
kanoate-based microbial scaffolds HA scaffold showed enhanced cell viability, proliferation, and differentiation, and could
for bone tissue engineering. Int J thus serve as a versatile platform for bone tissue engineering applications.
Bioprint. 2024;10(2):1806.
doi: 10.36922/ijb.1806
Received: September 12, 2023 Keywords: Polyhydroxyalkanoate; Biomineralization; Biopolymer; Bone scaffold;
Accepted: November 2, 2023 Polydopamine; Bioprinting
Published Online: January 16, 2024
Copyright: © 2024 Author(s).
This is an Open Access article
distributed under the terms of the 1. Introduction
Creative Commons Attribution
License, permitting distribution, There has been growing interest in biodegradable polymers as a substitute for traditional
and reproduction in any medi-
1–3
um, provided the original work is petrochemical-based polymers, with a focus on green alternative. The use of biopolymers
properly cited. is necessary in certain circumstances, such as for biomedical applications, due to their
4–6
Publisher’s Note: AccScience biodegradability, biocompatibility, and non-toxicity. Polyhydroxyalkanoates (PHAs)
Publishing remains neutral with are biodegradable polymers that are naturally produced in bacterial fermentation. PHAs
regard to jurisdictional claims in are composed of monomers derived from carboxylic acids, such as fatty acids, and their
published maps and institutional
affiliations. physical and chemical properties can vary depending on the specific types of monomers

Volume 10 Issue 2 (2024) 489 doi: 10.36922/ijb.1806