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International Journal of Bioprinting Bottom-up and top-down VAT photopolimerization

Figure 1. (a) CAD images of the bioprinter showing the (i) bottom-up and (ii) top-down configurations. (b) Schematics showing the setup for selecting
top-down and bottom-up configurations. (c) Schematics showing the bioprinter projection setup. (d) Microfluidics setup for on-a-chip bioprinting. (e)
Images of the PMMA mold, the casted PDMS chip, and the chip assembly. (f) Illustration of the bioprinting and the washing process between different
biomaterials.

photocrosslinkable solution of (poly (ethylene glycol) in Dulbecco’s phosphate-buffered saline (DPBS). GelMA
diacrylate (PEGDA); Mn = 700; Sigma-Aldrich, St. Louis, and PEGDA were used with different concentrations and
MO, USA) along with gelatin methacryloyl (GelMA) different photoinitiator (PI) concentrations. For evaluating
were used. GelMA was prepared by dissolving porcine cell printability, we used GelMA, which has been widely
skin gelatin (CAS number 9000-70-8; Sigma-Aldrich, St. used in various biomedical applications due to its suitable
Louis, MO, USA) and methacrylic anhydride (MA) (CAS biological properties and tunable physical characteristics.
number 760-93-0; Sigma-Aldrich, St. Louis, MO, USA) Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP)

Volume 10 Issue 2 (2023) 534 doi: 10.36922/ijb.1017