Page 172 - IJB-10-3

International Journal of Bioprinting 3D bone: Current & future

Table 6. 3D-bioprinted bone in vivo experiments
Experiment Materials Cells Printing Reference
technique
3D-printed Ch/MSC-laden grafts consisting of Alginate hMSCs; hChs DIW 99
alginate and BCP transplanted into female nude
mice
3D-printed β-TCP scaffolds implanted into rats β-TCP; pluronic Primary bone marrow Unspecified 100
MSCs
3D-bioprinted PCL-reinforced gene-activated RGD-γ-irradiated alginate and hMSCs FDM 101
bioinks for bone tissue engineering nHA complexed to pDNA; PCL
Nanoclay-based 3D-printed scaffolds implanted Alginate-methylcellulose bioink hBMSCs; HUVECs Extrusion-based 102
into nude mice
Calvarial defect model in DM rats GelMA; gelatin; PEG; MSNs BMSCs DIW 103
Calvarial bone reconstruction in rats PCL; TCP; unspecified hydrogel hAFSCs DIW 104
components
Pre-vascularized bone tissue implanted into mice Fibrin; gelatin; HA; glycerol; HUVECs; ASCs DIW 105
hydroxyapatite; VEGF; bFGF;
aprotinin
Porous bioprinted constructs in BMP-2 nonviral Alginate MG-63 cells; goat DIW 106
gene therapy for bone tissue engineering in the MSCs
dorsal pockets of mice
Critical-size rat calvarial defect treatment with ECM; amorphous magnesium DPSCs Inkjet 107
ECM/magnesium phosphate-based 3D-printed phosphate
craniomaxillofacial bone tissue
In vivo tibia repair and ectopic osteoinduction PEGDA; Laponite XLG ROBs DIW 108
experiments nanoclay; HA sodium salt
Hyperelastic bone scaffolds with MSCs implants in Hyperelastic bone MSCs DIW 55
mouse and rat
Bilateral calvarial bone defect in mice Collagen; HA nanoparticles Mesenchymal stromal LAB 109
cells
Subcutaneous implantation in mice Alginate; gelatin; collagen DPSCs DIW 110
Chitosan-based scaffolds implanted in nude mice Chitosan; SiO nanoparticles Human osteoblasts 111
2
for osteogenesis and vascularization
Calcium phosphate scaffold seeded with MSCs in Calcium phosphate paste Rat MSCs DIW 112
rats
β-TCP-seeded MSC and HUVEC scaffolds in rats β-TCP Mouse bone MSCs; DIW 113
HUVECs
Pre-vascularized PCL-hydrogel-cell 3D-printed PCL; fibrin; modified alginate; HUVECs; hBMSCs DIW 114
bone implanted into rats GelMA
Hybrid scaffolds with HUVECs and MSCs PCL; fibrin HUVECs; hMSCs Extrusion-based 115
implanted into rats
Micro-channeled MSC-GelMA scaffolds implanted GelMA; pluronic MSCs DIW 116
into rats

Abbreviations: ASC: Adipose-derived stem cell; BCP: Biphasic calcium phosphate particle; bFGF: Basic fibroblast growth factor; BMP-2: Bone
morphogenetic protein 2; BMSCs: Bone marrow stromal cells; DIW: Direct ink writing; DM: Diabetes mellitus; DPSC: Dental pulp stem cells; ECM:
Extracellular matrix; FDM: Fused deposition modeling; GelMA: Gelatin methacryloyl; HA: Hyaluronic acid; hAFSCs: Human amniotic fluid stem cells;
hBMSC: Human bone marrow stromal cells; hChs: Human articular chondrocytes; hMSC: Human mesenchymal stem cell; HUVECs: Human umbilical
vein endothelial cells; LAB: Laser-assisted bioprinting; MSC: Mesenchymal stem cell; MSN: Mesoporous silica nanoparticles; nHA: Nanohydroxyapatite;
PCL: Polycaprolactone; pDNA: Plasmid DNA; PEG: Polyethylene glycol; PEGDA: Polyethylene glycol diacrylate; RGD: Radiation generating device;
ROBs: Rat osteoblasts; TCP: Tricalcium phosphate; VEGF: Vascular endothelial growth factor.

Volume 10 Issue 3 (2024) 164 doi: 10.36922/ijb.2056