Page 192 - IJB-10-3

International Journal of Bioprinting Bioprinted tissue-on-a-chip in drug screening

Table 1. Summarized previous disease/organ-on-a-chip involving 3D bioprinting
Materials for
Bioprinting Hydrogel bioinks fabrications of Organ or tissue Culture duration Characteristics Reference
technology and functions
microfluidic parts
• Collagen
encapsulates
Extrusion HepG2 PCL Liver 6 days One-step bioprinting 118
with no additional
bioprinting • Gelatin adhesion
encapsulates
HUVECs
• The liver dECM
encapsulates
Extrusion HepaRG cells PEVA, PMMA Liver and biliary 7 days The introduction of 119
biliary tract enabling
bioprinting • Gelatin passage crosstalk between organs
encapsulates
HUVECs
The GelMA and
alginate blend
Extrusion encapsulates Cardiomyocytes in this
construct spontaneously
(coaxial) HUVECs PDMS, PMMA Heart 15 days contracting in one 120
bioprinting and adheres direction
cardiomyocytes after
consolidation

The gelatin and
Extrusion fibrin blend act as Kidney 7 days The connection between 121
blood vessels and renal
bioprinting ECM to adhere to tubes
cells
dECM derived from
porcine tracheal The interaction of airway
Extrusion mucosa encapsulates PCL, PDMS Airway 3 months epithelial and endothelial 122
bioprinting
endothelial cells and cells
fibroblasts
• Gelatin
encapsulates
hepatic stellate
Extrusion cells and PEVA Liver fibrosis The accurate deposition 127
bioprinting HUVECs of three cell types
• The liver dECM
encapsulates
HepaRG cells
• Collagen
encapsulates
Extrusion neuroblastoma PMMA Neuroblastoma 14 days Endothelial cells were 128
distributed evenly by an
bioprinting tumor spheroids orbital oscillator
• GelMA supports
the structure
The GelMA and
Extrusion gelatin blend Thrombus in the
(sacrificial) encapsulates PDMS Thrombosis 14 days perfusion state via a 129
bioprinting branch model
fibroblasts
Abbreviations: ECM, extracellular matrix; GelMA, gelatin methacrylate; HepG2, human hepatocellular carcinoma; HUVECs, human umbilical vein
endothelial cells; PCL, polycaprolactone; PEVA, poly-(ethylene/vinyl acetate); PMMA, polymethylmethacrylate; PDMS, polydimethylsiloxane.

Volume 10 Issue 3 (2024) 184 doi: 10.36922/ijb.1951