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International Journal of Bioprinting 3D-bioprinted hydrogel for pulp regeneration

gene expression to support robust cell self-renewal and To further analyze the aforementioned results, DLP-
stimulate the secretion of regeneration-associated bioactive based 3D-bioprinted DPGCs were fabricated to evaluate
factors, 37,40 which is beneficial for dental pulp regeneration the odontogenesis of hDPSCs cultured in an odontogenic
mediated by dental pulp stem cells. induction medium. ALP plays a critical role in the early

Figure 4. DLP-based 3D-bioprinted DPGCs improve the odontogenesis of hDPSCs in vitro. (A) ALP activity quantification and (B–F) odontogenesis-
related gene expression of hDPSCs encapsulated in DPGCs and bulk GelMA hydrogel constructs after culturing for 7 and 14 days. *p < 0.05, **p < 0.01,
***p < 0.001.

Volume 10 Issue 3 (2024) 309 doi: 10.36922/ijb.1790