Page 356 - IJB-10-3

International Journal of Bioprinting hNVU chip for brain modeling and drug screening

Figure 3. Characterization of EC intercellular junction proteins and evaluation of BBB function in the hNVU chip. (A, B) Representative
immunocytochemistry analysis of CD144 (adherens junction) and ZO1 (tight junctions) deposition by hCMEC/D3 cells. Scale bar: 50 μm. (C, D) qRT-
PCR analysis of genes associated with BBB integrity and BBB metabolic functions and transporters. (E) Permeability coefficients for three different
dextrans. (F) Permeability coefficients of 10 kDa FTIC–dextrans at three different detection times (days 3, 5, and 7). (G) TEER measurement of the BBB. n
= 3, independent experiments. Data are presented as the mean ± S.D. Significant differences between the groups were determined by t-test.

3.2. The brain region of the hNVU chip recapitulates pericytes, and iPSC-NPCs capable of differentiating into
the microenvironment of NVU cells in brain neuronal cells. The simulated brain model consists of a
Based on the BBB structure, we constructed a brain diverse cellular composition, including neural stem cells,
region structure in the middle of the sandwich structure. recapitulating the pathological cell microenvironment and
This structure included astrocytes, microglias, ECs, pathological process of microangiogenesis in brain.

Volume 10 Issue 3 (2024) 348 doi: 10.36922/ijb.1684