Page 407 - IJB-10-3

International Journal of Bioprinting In situ thermal monitoring in bioprinting

Figure 4. Monitoring system and printbed acquisition scheme. Under normal circumstances, the bioprinted construct is deposited on a sterilized substrate
(glass petri dish in this case) positioned upon the printbed for better temperature control.

to VR images, as a top-view solution was available in this image thresholding methods outperformed others in
case. The function mat2gray was used for each frame of terms of quantitative metrics. Whether applied to visible
the series, to rescale the pixel intensity linearly between or thermal images, this method consistently yielded
0 and 1. This was done because even if HD images were superior results, showcasing its robustness in image
characterized by an 8-bit integer value pixel-wise, the IR reconstruction. Moreover, when considering the utility of
camera provided a radiance value on a continuous scale. image thresholding, particularly in the context of VR and
IR images, its appeal lies in its simplicity and effectiveness.
2.7. Bradley adaptive thresholding algorithm While more complex segmentation methods exist, such as
Segmentation is a diverse and dynamic field within region-growing, edge detection, or deep learning-based
computer vision and image processing, characterized by techniques, image thresholding offers a straightforward
a wide range of algorithms and approaches tailored to and computationally efficient solution. Thus, the choice to
various applications and imaging modalities. In previous use image thresholding over more complex methods in this
internal experimentations, we conducted extensive testing context was driven by the need for simplicity and speed.
of both supervised and unsupervised algorithms, aiming
to identify the most effective method for achieving In global thresholding, a single threshold value
reconstructions with less error in both thermal and is applied uniformly to the entire image, which may
visible image datasets. Despite the diverse algorithms lead to suboptimal results when dealing with images
employed, our analyses consistently demonstrated that characterized by non-uniform lighting conditions or

Volume 10 Issue 3 (2024) 399 doi: 10.36922/ijb.2021