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International Journal of Bioprinting Printing collagen type IV membrane

3D printing tests, and Dr Monika Valtink (Institute of Consent for publication
Anatomy, Faculty of Medicine, TU Dresden, Germany)
for her generosity in offering the B4G12 cell line to our Doner corneas were used in this study, where all consents
laboratory. The authors acknowledge the facilities and were administrated through the New South Wales
the technical and scientific assistance of the Electron Tissue Bank. With ethics approval, the bank allocates
Microscopy Centre, University of Wollongong. We thank the corneas (that are not suitable for transplantation)
the Australian National Fabrication Facility (ANFF based on the consent they received from research groups.
Materials Node), Translational Research Initiative for Research groups do not have direct contact with human
Cell Engineering and Printing (TRICEP), and Australian subjects. Only de-identified information is allowed to be
Research Council (ARC) Centre of Excellence Scheme for published. No identifiable information was published in
using their facilities at the University of Wollongong. this paper.

Funding Availability of data

We acknowledge the funding support from the NHMRC Data can be obtained by contacting the corresponding
Idea Grant (GNT1181415), Australian Research Council author. No identifiable information about donor cornea
(ARC) Centre of Excellence Scheme (CE140100012), and will be made available to anyone other than the New South
Sydney Eye Hospital Foundation. Wales Tissue Bank.

Conflict of interest Further disclosure
The authors declare the following financial interests/ Part of the findings have been presented in the ANZ
personal relationships, which may be considered as Corneal Society in 2023, the International Society for
potential competing interests: the printable Col-IV Eye Research in 2023, and the Association for Research
biomaterial (PCT/AU2022/050247) was also patented in Vision and Ophthalmology (ARVO) Annual Meeting
with M.O., G.W., G.S., and J.Y. being inventors. The in 2023.
bioengineered endothelium construction was patented
(PCT/AU2022/050248) with M.O., S.H., L.W., C.P., G.S., References
and J.Y. being inventors.
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Conceptualization: Gerard Sutton, Jingjing You doi: 10.3389/fbioe.2019.00166
Formal analysis: Morgan Overmass, Huasheng Huang, 2. Song Y, Overmass M, Fan J, et al. Application of Collagen I
Jingjing You and IV in bioengineering transparent ocular tissues. Review.
Investigation: Morgan Overmass, Huasheng Huang Frontiers in Surgery. 2021-August-26 2021;8(344).
Methodology: Li Wen, Zhi Chen, Constantinos Petsoglou, doi: 10.3389/fsurg.2021.639500
Sepidar Sayyar, Li Wen 3. Dean A, Van Agtmael T. Collagen IV-related diseases and
Project administration: Jingjing You therapies. In: Ruggiero F, ed. The Collagen Superfamily
Resources: Gordon Wallace, Gerard Sutton, Jingjing You and Collagenopathies. Switzerland: Springer International
Supervision: Gerard Sutton, Jingjing You Publishing; 2021:143-197.
Validation: Constantinos Petsoglou, Gerard Sutton 4. De Santis MM, Wagner DE. Collagen IV: a critical new
Writing – original draft: Morgan Overmass, Jingjing You starting point for engineering upper airways. Eur Respir J.
Writing – review & editing: Morgan Overmass, Huasheng Jun 2020;55(6).
Hua, Li Wen, Sepidar Sayyar, Constantinos Petsoglou, doi: 10.1183/13993003.01130-2020
Gordon Wallace, Gerard Sutton, Jingjing You
5. Soh YQ, Kocaba V, Pinto M, Mehta JS. Fuchs endothelial
Ethics approval and consent to participate corneal dystrophy and corneal endothelial diseases: East
meets West. Eye (Lond). Mar 2020;34(3):427-441.
Primary human corneal endothelial cells (P-HCECs) were doi: 10.1038/s41433-019-0497-9
used in this study. P-HCECs were isolated from donated 6. Deng SX, Lee WB, Hammersmith KM, et al. Descemet
human corneas unsuitable for transplantation, obtained membrane endothelial keratoplasty: safety and outcomes:
from New South Wales Tissue Bank (NSWTB) with a report by the American Academy of Ophthalmology.
ethics approval (HREC 13/1041). All procedures were in Ophthalmology. Feb 2018;125(2):295-310.
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Volume 10 Issue 4 (2024) 169 doi: 10.36922/ijb.3258