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International Journal of Bioprinting 3D-Printed scaffolds for diabetic bone defects

Figure 4. Effects of PCL@SS31@E7 porous scaffolds on the regulation of mitochondrial function and osteogenic differentiation of BMSCs in high-glucose
microenvironment. (A, B) Impaired mitochondrial function, diminished osteogenic differentiation, and reduced gene and protein expression of ATP5A,
type I collagen, and OPN in BMSCs in the high-glucose microenvironment compared with normal medium (n = 3). (C, D) Immunofluorescence staining
of intracellular type I collagen in BMSCs under normal- and high-glucose environment culture conditions and quantitative analysis (n = 3). (E–L) Results
of quantitative RT-PCR for ATP5A, type I collagen, and OPN mRNA expression (E, n = 3), ALP activity assay (F, n = 3), immunofluorescence staining and
quantitative analysis of type I collagen and OPN (G–J, n = 3), and Alizarin Red staining and quantitative analysis (K and L, n = 3) performed in BMSCs after co-
cultivation with four different porous scaffolds in a high-glucose environment. (M) Schematic representation of the mechanism by which SS31 and E7 peptides
improve cellular recruitment and mitochondrial respiratory function of BMSCs in a high-glucose environment. NS, no significance; *P < 0.05; **P < 0.01.

Volume 10 Issue 4 (2024) 213 doi: 10.36922/ijb.2379