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International Journal of Bioprinting 3D-Printed scaffolds for diabetic bone defects

Figure 3. In vitro biocompatibility, adhesion, and migration. (A) Live/dead staining assay of BMSCs co-cultured with four PCL porous scaffolds for 48 h.
Live cells are stained green, while dead cells are red (scale bar: 100 μm). (B) CCK-8 assay for cell proliferation of BMSCs co-cultured with different PCL
porous scaffold extracts for 1–3 days (n = 5). (C) Attachment of BMSCs to four different PCL scaffolds after 48 h of co-culture (scale bar: 50 μm). (d)
SEM images of four PCL porous scaffolds co-cultured with BMSCs after 48 h (scale bar: 50 μm); (E, F) Cell migration from 0 to 24 h in the BMSC scratch
assay and quantitative analysis of cell mobility (n = 3). (H–K) Assessment of cell chemotaxis: microscopic images of the migration of porous scaffold from
the upper chamber (H); schematic diagram of the chemotaxis experiment (I, J); and quantification of the number of migrating cells (K) (n = 3). NS, no
significance; *P < 0.05; **P < 0.01.

Volume 10 Issue 4 (2024) 212 doi: 10.36922/ijb.2379