Page 412 - IJB-10-4

International

Journal of Bioprinting

RESEARCH ARTICLE
Investigating the effect of pregabalin on

neuronal development using ultrashort
self-assembling peptides: Assessing

3D neuronal cultures with high throughput
robotic 3D bioprinting

Walaa F. Alsanie * , Sherin Abdelrahman 3,4,5† id , Majid Alhomrani 1,2 id ,
1,2 ,† id
Alexander U. Valle-Pérez 3,4,5 id , Ebtisam Abdulah Alosimi 2 id ,
Hamza Habeeballah 6 id , Heba A. Alkhatabi 7,8,9 id , Raed I. Felimban 7,10 id ,
Abdulhakeem S. Alamri 1,2 id , Abdulaziz Alsharif 1 id , Bassem M. Raafat 11 id ,
*
Yusuf S. Althobaiti 12,13 id , Ahmed Gaber 2,14 id , and Charlotte A. E. Hauser 3,4,5 id
1 Department of Clinical Laboratories Sciences, The Faculty of Applied Medical Sciences, Taif
University, Taif, Saudi Arabia
2 Centre of Biomedical Sciences Research (CBSR), Deanship of Scientific Research, Taif University,
Taif, Saudi Arabia
3
Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering
(BESE), King Abdullah University of Science and Technology (KAUST), Jeddah, Saudi Arabia
† These authors contributed equally 4 Computational Bioscience Research Center (CBRC), KAUST, Jeddah, Saudi Arabia
to this work. 5 Red Sea Research Center (RSRC), KAUST, Jeddah, Saudi Arabia
*Corresponding authors: 6 Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences in Rabigh,
Walaa F. Alsanie King Abdulaziz University, Jeddah, Saudi Arabia
(w.alsanie@tu.edu.sa)
7 Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz
Charlotte A. E. Hauser University, Jeddah, Saudi Arabia
(chauser.noltenius@gmail.com)
8 Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah,
Citation: Alsanie WF, Saudi Arabia
Abdelrahman S, Alhomrani M, et al. 9
Investigating the effect of pregabalin King Fahd Medical Research Centre, Hematology Research Unit, King Abdulaziz University,
on neuronal development using Jeddah, Saudi Arabia
ultrashort self-assembling 10 Center of Innovation in Personalized Medicine (CIPM), 3D Bioprinting Unit, King Abdulaziz
peptides: Assessing 3D neuronal University, Jeddah, Saudi Arabia
cultures with high throughput
robotic 3D bioprinting. 11 Department of Radiological Sciences, College of Applied Medical Sciences, Taif University, Taif,
Int J Bioprint. 2024;10(4):3010. Saudi Arabia
doi: 10.36922/ijb.3010 12 Department of Pharmacology and Toxicology, College of Pharmacy, Taif University, Taif, Saudi
Arabia
Received: February 25, 2024
Accepted: May 13, 2024 13 Addiction and Neuroscience Research Unit, Taif University, Taif, Saudi Arabia
Published Online: July 18, 2024 14 Department of Biology, College of Science, Taif University, Taif, Saudi Arabia
Copyright: © 2024 Author(s).
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution, Abstract
and reproduction in any medium,
provided the original work is Pregabalin is a widely prescribed drug for various neurological disorders, yet its effects
properly cited. on embryonic cortical neuron development when given to pregnant women remain
Publisher’s Note: AccScience inadequately explored. In this study, we employed advanced three-dimensional
Publishing remains neutral with (3D) culturing and in-house developed high-throughput robotic 3D bioprinting
regard to jurisdictional claims in
published maps and institutional technologies to evaluate their potential in neuropharmacology applications, using
affiliations. pregabalin as a model compound. Using a robotic 3D bioprinter and tetrameric IIZK

Volume 10 Issue 4 (2024) 404 doi: 10.36922/ijb.3010