Page 483 - IJB-10-4

International

Journal of Bioprinting

RESEARCH ARTICLE
Development of embedded bioprinting for

fabricating zonally stratified articular cartilage

Yang Wu * , Xue Yang , Tianying Yuan , Seung Yeon Lee , Minghao Qin ,
1
3
1
4
1,2 id
Sung Jun Min , Bingxian Lu , Pengkun Guo , Jiarui Xie , Shengli Mi *,
4
3,5
1
1
1
and Dong Nyoung Heo *
6,7
1 School of Mechanical Engineering and Automation, Harbin Institute of Technology, Shenzhen,
China
2 Guangdong Provincial Key Laboratory of Intelligent Morphing Mechanisms and Adaptive Robotics,
Harbin Institute of Technology, Shenzhen, China
3
Biomanufacturing Engineering Laboratory, Tsinghua Shenzhen International Graduate School,
Shenzhen, China
4 Department of Dentistry, Graduate School, Kyung Hee University, Seoul, Republic of Korea
5 Open FIESTA Center, Tsinghua Shenzhen International Graduate School, Shenzhen, China
6 Department of Dental Materials, School of Dentistry, Kyung Hee University, Seoul, Republic of
Korea
7 Biofriends Inc., Seoul, Republic of Korea
(This article belongs to the Special Issue: 3D printing of bioinspired materials)
*Corresponding authors:
Yang Wu
(wuyang2019@hit.edu.cn) Abstract
Shengli Mi
(mi.shengli@sz.tsinghua.edu.cn) Embedded bioprinting enables direct deposition of bioinks in three dimensions
Dong Nyoung Heo inside a support bath with shear-thinning and self-healing capabilities, and it
(heodaeng@khu.ac.kr) has been used to fabricate complex tissues and organs for several biomedical
Citation: Wu Y, Yang X, Yuan T, applications. In this study, a support bath comprising gelatin/alginate microparticles

et al. Development of embedded and oxidized alginate solution was developed and crosslinked in situ with carbonyl
bioprinting for fabricating zonally
stratified articular cartilage. hydrazide-modified gelatin bioink via the Schiff base reaction. The numerical
Int J Bioprint. 2024;10(4):3520. model of embedded printing was established to analyze the extrusion process
doi: 10.36922/ijb.3520 and disturbance of the support bath. The process window (e.g., extrusion pressure,
Received: April 28, 2024 nozzle moving speed, nozzle size, and support bath composition) was established
Accepted: June 4, 2024 experimentally to ensure stable fiber formation. In addition, the compressive
Published Online: July 15, 2024
modulus of the printed construct has been reinforced due to the formation of
Copyright: © 2024 Author(s). interpenetrating polymer networks in the microparticles. Based on the process
This is an Open Access article investigation, a zonally stratified artificial cartilage with a three-layered structure was
distributed under the terms of the
Creative Commons Attribution designed: vertically printed fibers in the bottom, oblique fibers in the middle, and
License, permitting distribution, horizontally printed fibers in the superficial layer. The bioprinted cartilage supported
and reproduction in any medium, cell survival, proliferation, and spreading, with the observed deposition of cartilage-
provided the original work is
properly cited. specific proteins, offering a new strategy for developing tissue-engineered cartilage
constructs with biological and histological relevance.
Publisher’s Note: AccScience
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: Embedded bioprinting; Printability; Zonally stratified cartilage;
affiliations. Schiff base; Interpenetrating polymer network

Volume 10 Issue 4 (2024) 475 doi: 10.36922/ijb.3520