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International Journal of Bioprinting 3D printed hydrogels for tumor therapy

that HAp/GelMA hydrogels showed excellent mechanical significantly improved the printability of inks and
strength and could facilitate osteogenic differentiation in enhanced the mechanical strength of resulting hydrogels.
vitro and accelerate the formation of new bone in rabbit Moreover, 3D-printed MgHAp/GelMA-PDA@DOX
skull defects in vivo. Furthermore, previous studies have hydrogels exhibited a good photothermal effect and
34
revealed that magnesium (Mg)-doped HAp (MgHAp) enabled sustainable and controllable release of DOX. In
displayed a superior osteogenic effect in comparison to vitro experiments demonstrated that 3D-printed MgHAp/
HAp, owing to the sustained release of Mg ions. The GelMA-PDA@DOX hydrogels could be combined with
2+
release of Mg enabled to modulate bone metabolism, chemotherapy and PTT and hence effectively eradicate
2+
regulate osteoblast and osteoclast activity, and stimulate MG63 osteosarcoma cells. Compared to HAp/GelMA-
new bone formation. 35,36 Consequently, compared to HAp/ PDA hydrogels, MgHAp/GelMA-PDA hydrogels promoted
GelMA hydrogels, 3D-printed MgHAp/GelMA hydrogels proliferation and osteogenic differentiation of rat bone
may potentially provide an enhanced therapeutic efficacy marrow-derived mesenchymal stem cells (rBMSCs) in
for the treatment of bone defects. vitro owing to the sustained release of Mg . Therefore,
2+
In the current study, to build an on-site controlled 3D-printed MgHAp/GelMA-PDA@DOX hydrogels hold
drug release system that can induce hyperthermia great potential for both cancer therapy and bone tissue
and modulate drug release for eradicating tumor cells regeneration, which is desirable for the treatment of bone
and simultaneously promote bone tissue regeneration, tumor-related defects.
MgHAp/GelMA hydrogels encapsulated with PDA@DOX 2. Materials and methods
particles (MgHAp/GelMA-PDA@DOX) were fabricated
via 3D printing (Figure 1). PDA particles encapsulated 2.1. Materials
with an anticancer drug, doxorubicin hydrochloride Type I collagen (COL1), citric acid, gelatin (Gel; type
(DOX), were synthesized and then incorporated in A from porcine skin, powder, gel strength ~300 g
MgHAp/GelMA hydrogels. The incorporation of MgHAp Bloom), methacrylic anhydride (MA; 94%), 2-hydroxy-

Figure 1. Schematic illustration of the fabrication of MgHAp/GelMA-PDA@DOX hydrogels using 3D printing and their dual functionality in eradicating
tumor cells and promoting bone tissue regeneration.

Volume 10 Issue 5 (2024) 234 doi: 10.36922/ijb.3526