Page 240 - IJB-10-5

International

Journal of Bioprinting

RESEARCH ARTICLE
3D-printed Mg-substituted hydroxyapatite/

gelatin methacryloyl hydrogels encapsulated
with PDA@DOX particles for bone tumor therapy

and bone tissue regeneration

Shangsi Chen 1† id , Yue Wang 1,2† , Junzhi Li , Haoran Sun *,
3†
4
Ming-Fung Francis Siu *, and Shenglong Tan *
2
5 id
1 Department of Mechanical Engineering, Faculty of Engineering, The University of Hong Kong,
Pokfulam Road, Hong Kong, China
2 Department of Building and Real Estate, Faculty of Construction and Environment, The
Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China
3
Department of Surgery, School of Clinical Medicine, The University of Hong Kong, Pokfulam
Road, Hong Kong, China
4 Koln 3D Technology (Medical) Limited, Hong Kong Science Park, Shatin, Hong Kong, China
5 Department of Endodontics, Stomatological Hospital, Southern Medical University, Guangzhou,
China
† These authors contributed equally
to this work. (This article belongs to the Special Issue: Advanced Biomaterials for 3D Printing and Healthcare Application)
*Corresponding authors:
Haoran Sun
(haoran@koln3d-tech.com) Abstract
Ming-Fung Francis Siu
(francis.siu@polyu.edu.hk) The development of bifunctional scaffolds for clinical applications, aimed at
Shenglong Tan preventing tumor recurrence and promoting bone tissue regeneration simultaneously
(tansl1989@outlook.com) at the surgical site, is imperative in repairing bone tumor-related defects. In the
current study, Mg-substituted hydroxyapatite (MgHAp) nanocomposites were
Citation: Chen S, Wang Y, Li J,
Sun H, Siu MF, Tan S. 3D-printed synthesized via a biomineralization process. Doxorubicin hydrochloride (DOX), an

Mg-substituted hydroxyapatite/ anticancer drug, was incorporated in polydopamine (PDA) particles to synthesize
gelatin methacryloyl hydrogels PDA@DOX particles. MgHAp/gelatin methacryloyl (GelMA) hydrogels encapsulated
encapsulated with PDA@DOX
particles for bone tumor therapy and with PDA@DOX particles were designed and fabricated to construct MgHAp/GelMA-
bone tissue regeneration. PDA@DOX hydrogels via 3D printing. The 3D-printed MgHAp/GelMA-PDA@DOX
Int J Bioprint. 2024;10(5):3526. hydrogels exhibited antitumor synergy by providing combined chemotherapy
doi: 10.36922/ijb.3526
and phototherapy for bone tumor cell ablation. The hydrogels showed a good
Received: April 29, 2024 photothermal effect and could induce hyperthermia upon irradiation with an 808
Accepted: May 14, 2024
Published Online: July 17, 2024 nm near-infrared (NIR) laser. Moreover, MgHAp/GelMA-PDA@DOX hydrogels could
release DOX sustainably and controllably. In vitro experiments demonstrated that
Copyright: © 2024 Author(s).
This is an Open Access article 3D-printed MgHAp/GelMA-PDA@DOX hydrogels could effectively eradicate MG63
distributed under the terms of the cells through the synergy of induced hyperthermia and DOX release. Furthermore,
Creative Commons Attribution due to the sustained release of Mg , 3D-printed MgHAp/GelMA-PDA@DOX hydrogels
2+
License, permitting distribution,
and reproduction in any medium, could promote the proliferation of rat bone marrow-derived mesenchymal stem cells
provided the original work is and facilitate alkaline phosphatase activity and the expression of osteogenic genes,
properly cited. such as osteocalcin (Ocn), type I collagen (Col1), runt-related transcription factor-2
Publisher’s Note: AccScience (Runx2), and bone morphogenetic protein-2 (Bmp2), indicating their excellent
Publishing remains neutral with osteogenic effect. As a result, 3D-printed MgHAp/GelMA-PDA@DOX hydrogels
regard to jurisdictional claims in showed great potential in the treatment of bone tumor-related defects by effectively
published maps and institutional
affiliations. killing tumor cells and simultaneously promoting bone tissue regeneration.

Volume 10 Issue 5 (2024) 232 doi: 10.36922/ijb.3526