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International Journal of Bioprinting Printing organoids in peptide matrices

Figure 2. Culture, viability and proliferation evaluation of SW1222 cells in decorated self-assembling peptide scaffolds. (a) Brightfield images of colorectal
cancer (CRC) organoids generated in different matrices and 2D control. Scale bars: 150 m. (b) Cell viability in different matrices and 2D control. Live
cells were stained with calcein-AM (green), and dead cells were stained with ethidium homodimer-1 (red). Scale bars: 150 µm. (c) Influence of different
matrices on CRC cell proliferation assay at days 1, 4, and 7 of culture (n = 20). Matrigel (Mat) is considered a positive control, and the 2D control (2D) is
considered a negative control. A t-test was conducted between days 1 and 7 for each single condition. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
Abbreviations: FIB, fibrinogen-derived peptide containing the RGD motif; LAM, laminin-derived peptide containing YIGSR.

Volume 10 Issue 5 (2024) 349 doi: 10.36922/ijb.3033