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International Journal of Bioprinting Printing organoids in peptide matrices

Figure 6. Colorectal cancer (CRC) organoids in different matrices with stained apical polarity markers (ZO1 and ezrin) on day 7. Ezrin expression is present
in parent (low) organoid cultures by day 7 and absent in parent (high) cultures. FIB (low) and Matrigel conditions display consistent apical and basal ezrin
localization, while LAM (high) exhibits a diffused organoid-wide distribution, diverging from its usual membrane localization. Results are based on three
technical replicates and two biological replicates. Positive (Matrigel) and negative (2D) controls are denoted with (+) and (−) signs, respectively. Scale bars:
100 μm. Abbreviations: FIB, fibrinogen-derived peptide containing the RGD motif; LAM, laminin-derived peptide containing YIGSR.

Volume 10 Issue 5 (2024) 354 doi: 10.36922/ijb.3033