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International Journal of Bioprinting Printing organoids in peptide matrices

Figure 5. Colorectal cancer (CRC) organoids in different matrices with stained apical polarity markers (ZO1 and ezrin) on day 4. Ezrin marker expression
becomes evident by day 4 across all experimental conditions, except the two parent peptides. Notably, ZO-1 expression in the Matrigel, LAM (high), and
FIB (low) samples display prominent localization to both the apical and basal epithelial surfaces of the organoid. Positive (Matrigel) and negative (2D)
controls are denoted with (+) and (−) signs, respectively. Scale bars: 100 μm. Abbreviations: FIB, fibrinogen-derived peptide containing the RGD motif;
LAM, laminin-derived peptide containing YIGSR.

Volume 10 Issue 5 (2024) 353 doi: 10.36922/ijb.3033