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International Journal of Bioprinting DEX-Loaded PLGA microspheres enhance cartilage regeneration

Figure 1. Schematic diagram of experiment flow. (A) Fabrication process of microspheres (MPs). (B) In vivo experiment process. Abbreviations: DEX,
dexamethasone; GelMA, gelatin methacryloyl; LAP, lithium phenyl-2,4,6-trimethylbenzoylphosphinate; MPs, microspheres; PLGA, poly (lactic-co-
glycolic acid).

were purchased from Xienshi Biological Technology Aldrich (Germany). TRIzol Reagent was acquired from
Co., Ltd. (Tianjin, China). Dulbecco’s Modified Eagle Invitrogen (USA).
Medium (DMEM), Roswell Park Memorial Institute
1640 medium (RPMI-1640), penicillin-streptomycin- 2.2. Animals
neomycin antibiotics (PSN), fetal bovine serum (FBS), The Japanese White rabbits (7-month-old female) and
and trypsin were obtained from Gibco (Grand Island, NY, C57 mice (6-week-old female) were purchased from
USA). Type II collagenase was purchased from Sigma- Long’an Experimental Animal Breeding Center (Beijing,

Volume 10 Issue 5 (2024) 385 doi: 10.36922/ijb.3396