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International Journal of Bioprinting Bioprinting for large-sized tissue delivery

Figure 4. 3D printing of large-sized architectures with high injection capacity. (A) Design of complicated models and (B) corresponding printed
architectures with disparate repetitive units and tunable Poisson’s ratios. (C) The injection of large-sized architectures through dispensing needles with
an inner diameter of 1.5 mm. (D) Design and corresponding printed architectures of the radiation sinusoid (radiation filling) and hexagonal lobule
models (60° linear filling). GP bioink is colored with red and blue dyes to distinguish the filaments printed by different nozzles. Scale bars: 5 mm (B–D).
Abbreviations: GP: Gelatin methacryloyl/poly(ethylene glycol) diacrylate; PPR: Positive Poisson’s ratio; ZPR: Zero Poisson’s ratio; and NPR: Negative
Poisson’s ratio.

activity were upregulated. In contrast, gene sets associated suitability, with greater sensitivity to environmental
with the differentiation and development of non-hepatic disturbances compared to immortalized or cancer
lineages were downregulated (Figure S12C, Supporting cell lines. 41
Information), suggesting improved hepatic functions After decoupled bioprinting, M14A maintained a
and heightened susceptibility to viral infection of M14A high cell viability of 95.3 ± 1.8%, indicating minor harm
compared to HepaRG. Meanwhile, the genome-edited cells from bioprinting and photocrosslinking to cells (Figure
emerged as robust candidates for studying cell-bioprinting 5E). RNA-sequencing analysis was conducted to compare

Volume 10 Issue 5 (2024) 437 doi: 10.36922/ijb.3898