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Magnetic levitational bioassembler
such as monolithic polycarbonate to provide airless locking mechanism that protects against accidental
filling of containers and the possibility of visual pressing of the piston systems (Figure 3D), which
control of the biofabrication process. The inner has a spring-loaded positioning mechanism in three
parts of the cuvette are shown in Figure 3B. Since positions (Figure 3E).
various fixatives, including paraformaldehyde, were It should be noted that the use of the magnetic
used in the studies, the design of the cell requires bioassembler “Organ.Aut” with cuvettes allows
three levels of biosafety. Accordingly, the layout of directed assembly of living biological constructs
the cuvette that consists of three assembled cases from tissue spheroids or various types of bacteria.
was developed (Figure 3C). In addition to the three Furthermore, it can be used to study the behavior
protection loops, the design of the cuvette includes a of crystalline forms of chemical compounds

A B G

C D

E F

Figure 2. The basic design and construction of “Organ.Aut.” (A) The framework, (B) the framework
with six pairs of cuvettes; (C) the assembly of two magnets; (D) the external view of illumination;
(E) the external view of the cover shelf; (F) the external view of Go Pro Hero 4 Silver camera; (G) the
blast-pattern of “Organ.Aut.”

A D F

B

E

C

Figure 3. The design of cuvette. (A) The scheme of cuvette structure; (B) the inner parts of cuvette;
(C) biosafety contours; (D) the protection from accidental hitting; (E) the fixation mechanism for
protection from accidental hitting; (F) the assembly of the cuvette.

114 International Journal of Bioprinting (2020)–Volume 6, Issue 3