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3D-printed bioreactors for in vitro modeling and analysis
in Tables 1 and 2. 3D-printing process can be design requirements of bioreactor, including
selected based on the manufacturing capability functionality and visual appearances [9,18] .
of the printer and the type of material used by Different laser sintering approaches such as
printer [20,21] . However, 3D-printing process can SLM and DMLS are highly reproducible AM
be also selected according to the given main techniques used to fabricate porous and 3D

Table 1. 3D-printed bioreactors used in mammalian cell culture applications for assessing of
cell viability, cell encapsulation, cell/tissue models, cell imaging, cell therapy, and organ-on-chip
applications.
Printing Printer model Possible reason Material 3D construct Application Cells used Ref.
technique for choice of developed
printer
Cell viability
SLA ILIOS High precision, PEG-DA-250 Transparent Bioreactor for Chinese [18]
3D-printer transparency resin disks studying resin hamster
compatibility on ovary cells
cells (CHO-K1),
Primary
hippocampal
neurons
SLA Form High accuracy, Photocurable Cylindrical test Bioreactor Zebrafish [17]
1+3D-printer high resolution liquid resin parts accessory for
FDM Dimension Affordable, ABS studying resin
Elite 3D-printer easily available compatibility on
cells
Material Objet350 Dimensional Objet Vero Microfluidic Bioreactor Bovine [31]
jetting Connex accuracy Clear chip for resin pulmonary
3D-printer compatibility on artery
cells endothelial
cells
Cell encapsulation
SLA Commercial High accuracy 3D Systems Pump-free Bioreactor for cell Oral [32]
3D-printer Accura® 60 perfusion cell encapsulation squamous
(Proto Labs) culture device cell
Material Objet260 Dimensional VeroClear- carcinoma
jetting Connex3 accuracy RGD810 tumor, Liver
3D-printer cells
Cell/tissue models
SLA Commercial Structural Eshell 300 Chamber and Bioreactor Human bone [33]
3D-printer robustness insert accessory for marrow stem
(EnvisionTEC) tissue interactions cell
Testing of therapeutics
Material Objet Connex Droplet Objet Vero Device Bioreactor for Endothelial [34]
jetting 350 3D-printer precision White Plus cell toxicity+drug cells
transport
Material Objet Connex Geometrical Objet Microfluidic Bioreactor for Red blood [13]
jetting 350 3D-printer precision VeroClear chip drug metabolism cells
Material MakerBot Good ABS Cartridges Accessory item Human [35]
extrusion Replicator 2X mechanical for cell toxicity embryonic
3D-printer properties kidney cells
(Contd...)

82 International Journal of Bioprinting (2020)–Volume 6, Issue 4