Page 182 - IJB-10-6

International

Journal of Bioprinting

RESEARCH ARTICLE
Electrospun polylactic acid-glycolic acid

composite hydrogel scaffold loaded with 3D
extracellular vesicles for nasal septal cartilage

defect repair

Jie Yang 1,2† id , Haolei Hu 2† id , Qiang Guo 3† id , Xiaolei Chen 1 id , Shuo Li , Gang Yin ,
2
1
Wei Yue , Yi Zhang ,Boxun Liu , Jianwei Chen * , Tao Xu * ,and Yi Li *
5
2
5
4 id
4 id
2 id
1 Department of Clinical, Faculty of Graduate Student, Xinxiang Medical University, Xinxiang City,
453003, Henan Province, China
2 Department of Otolaryngology, The 988th Hospital of the Joint Support Force of the Chinese
People’s Liberation Army, Zhengzhou City, 450042, Henan Province, China
3
Department of Urology, The Sixth Affiliated Hospital, Sun Yat-sen University, No 26 Yuancun
Erheng Road, Guangzhou, 540655, Guangdong, China
4 Bio-intelligent Manufacturing and Living Matter Bioprinting Center, Research Institute of Tsinghua
University in Shenzhen, Tsinghua University, Shenzhen, 518053, Guangdong, China
5 Department of Research and Development, Huaqing Zhimei (Shenzhen) Biotechnology Co., Ltd.,
† These authors contributed equally Shenzhen 518107, Guangdong, China
to this work. (This article belongs to the Special Issue: Bioprinting of Tissues and Organs: Materials, Technologies and
Affiliations 1 and 2 are the co-first Perspectives for Regenerative Medicine)
institutions
*Corresponding authors: Abstract
Jianwei Chen
(chenjw@tsinghua-sz.org) The nasal septum plays an important role in the growth and support of the human
Tao Xu nose, and defects can cause nasal deformities. Extracellular vesicles (EVs) have
(taoxu@mail.tsinghua.edu.cn) demonstrated great potential in tissue repair. Stem cell EVs are widely used in the repair
Yi Li (liyi153@aliyun.com)
of articular cartilage defects, but their use for nasal septal cartilage defects has not
Citation: Yang J, Hu H, Guo Q, been reported. Due to the low yield and loss of EVs during in situ injection, improved
et al. Electrospun polylactic
acid-glycolic acid composite preparation methods and better carriers are needed for the effective sustained release
hydrogel scaffold loaded with 3D of EVs in wounds. In this study, swelling and degradation experiments were initially
extracellular vesicles for nasal conducted on the scaffold, along with mechanical performance testing, including
septal cartilage defect repair.
Int J Bioprint. 2024;10(6):4118. observation of the scaffold morphology using scanning electron microscopy (SEM).
doi: 10.36922/ijb.4118 Subsequently, in vitro cell experiments were conducted to evaluate the ability of
3D EVs to promote chondrocyte proliferation, migration, and extracellular matrix
Received: July 2, 2024
Revised: August 11, 2024 formation. Finally, the EV-laden gelatin methacrylic acid-polylactic acid-glycolic
Accepted: August 19, 2024 acid (Gel-PLGA) composite scaffold was implanted into the nasal septum defect
Published Online: August 20, 2024
site of rabbits in vivo to observe its repair effect on the defect. In vitro experiments
Copyright: © 2024 Author(s). demonstrated that the biological scaffold exhibited good biocompatibility and could
This is an Open Access article effectively promote the proliferation and migration of chondrocytes. In vivo, the EV-
distributed under the terms of the
Creative Commons Attribution laden composite biological scaffold was implanted into the nasal septum defect
License, permitting distribution, of rabbits, and the tissues were tested at 6 and 12 weeks after surgery. The results
and reproduction in any medium, indicate that the composite scaffolds effectively facilitated the repair of defect sites.
provided the original work is
properly cited. Taken together, 3D EVs facilitate tissue repair and healing, offering a novel approach
to treating nasal septal defects.
Publisher’s Note: AccScience
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: 3D printing; Electrospinning; Hydrogel; Extracellular vesicles;
affiliations. Nasal septal defect

Volume 10 Issue 6 (2024) 174 doi: 10.36922/ijb.4118