Page 198 - IJB-10-6

International Journal of Bioprinting 3D-printed EVs for nasal septal defects

expertise in 3D coaxial printing combined with different Investigation: Jie Yang, Haolei Hu, Qiang Guo, Xiaolei
tissue cells to produce disease-specific 3D EVs is essential Chen, Shuo Li, Gang Yin, Wei Yue
for optimizing treatment outcomes. Methodology: Haolei Hu
Resources: Yi Zhang, Boxun Liu
In this experiment, we investigated the impact of EVs Writing - Original Draft: Jie Yang
derived from 3D coaxial printing on the repair of nasal Writing - Review & Editing: All authors
septal cartilage defects in rabbits. Our findings demonstrate
the significant potential of EVs in regenerative medicine. Ethics approval and consent to participate
Additionally, our combination of GelMA and PLGA with
3D EVs achieved successful repair within the body. Future The experimental animal ethics of this study have been
th
research should focus on identifying suitable biological reviewed by the Ethics Committee of the 988 Hospital of
materials for different anatomical locations to develop the Joint Logistics Support Force of the Chinese People’s
customized tissue repair therapies. Liberation Army (approval no. 988YY20230007LLSP;
approved project name: “Mechanism Study on Constructing
5. Conclusion MiR-920/BMSCs Biomimetic Stents for Repairing Rabbit
Nasal Septal Cartilage Defects”). All animal experiments
In this experiment, we have innovatively developed a (male rabbits) comply with the ARRIVE guideline and EU
composite biological scaffold by combining GelMA animal experimentation directives.
hydrogel and PLGA nanofiber. We have also successfully
prepared 3D EVs using 3D coaxial printing technology Consent for publication
and obtained a Gel-PLGA+EVs biological scaffold that
enables sustained release of EVs. The composite scaffold Not applicable
has demonstrated potential in accelerating cartilage defect
healing and promoting collagen deposition at the site. Availability of data
These findings indicate that the Gel-PLGA+EVs composite All relevant data are presented in the article. Other
scaffold holds great promise for clinical applications in data is available upon reasonable request from the
cartilage defect treatment. corresponding authors.

Acknowledgments References
The authors would like to thank all laboratory personnel at 1. Samibut P, Meevassana J, Suwajo P, et al. The anatomical study
Huaqing Zhimei Technology Co., Ltd. for their assistance of the nasal septal cartilage with its clinical implications.
with the study. Aesthetic Plast Surg. 2021;45:1705-1711.
doi: 10.1007/s00266-020-02116-z
Funding 2. Chiesa-Estomba CM, Aiastui A, González-Fernández I,

This study was researched by the Joint Construction et al. Three-dimensional bioprinting scaffolding for nasal
of Scientific and Technological Research Grant (No. cartilage defects: a systematic review. Tissue Eng Regen Med.
LHGJ20220916) and the Natural Science Foundation (No. 2021;18:343-53.
242300420125) of the Henan province, the 988 Hospital doi: 10.1007/s13770-021-00331-6
th
selects scientific research projects for key disciplines (No. 3. Setton LA, Elliott DM, Mow VC. Altered mechanics of
YNZX2024007), the Shenzhen Science and Technology cartilage with osteoarthritis: human osteoarthritis and an
Major Project of Shenzhen Science and Technology experimental model of joint degeneration. Osteoarthritis
Innovation Bureau (No. KJZD20230923114302006), Cartilage. 1999;7:2-14.
doi: 10.1053/joca.1998.0170
and the Guangdong Province key areas research and
development plan (No. 2023B0909020003). 4. Günebakan Ç, Kuzu S. Congenital deficiency of alar
cartilage. J Craniofac Surg. 2021;32:e137-8.
Conflict of interest doi: 10.1097/SCS.0000000000006858
5. Lavernia L, Brown WE, Wong BJF, Hu JC, Athanasiou
The authors have no competing interests.
KA. Toward tissue-engineering of nasal cartilages. Acta
Biomater. 2019;88:42-56.
Author contributions doi: 10.1016/j.actbio.2019.02.025
Conceptualization: Jianwei Chen, Tao Xu, Yi Li 6. Bagher Z, Asgari N, Bozorgmehr P, Kamrava SK, Alizadeh
Formal analysis: Jie Yang R, Seifalian A. Will tissue-engineering strategies bring

Volume 10 Issue 6 (2024) 190 doi: 10.36922/ijb.4118