Page 292 - IJB-10-6

International Journal of Bioprinting 3D-Printed Zn/MgHA-PCL for angio/osteogenesis

excellent mechanical properties and processability and osteogenic efficacy of co-doped HA-PCLs was validated
is a United States (US) Food and Drug Administration using a rat femoral defect model.
(FDA)-approved synthetic polymer widely used in bone
tissue repair. 35 2. Materials and methods
As displayed in Figure 1, this study initially synthesized 2.1. Materials
Zn-doped HA and Zn/Mg-co-doped HA via hydrothermal Calcium nitrate tetrahydrate (Ca(NO ) ·4H O, analytical
3 2
2
synthesis. Subsequently, 3D printing technology was reagent [AR]), magnesium nitrate hexahydrate
employed to fabricate element-doped HA-PCL composite (Mg(NO ) ·6H O, AR), zinc nitrate tetrahydrate
3 2
2
scaffolds for in vitro and in vivo angiogenesis and (Zn(NO ) ·4H O, AR), phosphoric acid dodecahydrate
3 2
2
osteogenesis studies. The angiogenic and osteogenic disodium hydrogen (Na₂HPO₄⋅12H₂O, AR), nitric
potential of co-doped HA-PCLs was confirmed via acid (HNO , AR), glutaraldehyde (C H O , AR), urea
3
2
5
8
human umbilical vein endothelial cells (HUVECs) and rat (CH N O, AR), and absolute ethanol (AR) were purchased
2
4
BMSCs (RBMSCs) activity assays in vitro. Meanwhile, the from Chengdu Kelong Chemical Reagent Factory (China).

Figure 1. Schematic diagram of the preparation and function of Zn/Mg@HA and Zn/Mg@HA-PCL in angiogenesis and osteogenesis. The figure was
created using BioRender (biorender.com). Abbreviations: BMSCs, bone marrow mesenchymal stem cells; HA, hydroxyapatite; HUVECs, human umbilical
vein endothelial cells; PCL, polycaprolactone.

Volume 10 Issue 6 (2024) 284 doi: 10.36922/ijb.4243