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International Journal of Bioprinting Collagen hydrolysate-loaded ODMA/PEGDMA scaffold

Figure 2. Illustration of the synthesis and extraction procedure of dopamine methacrylate (DMA).

2.4. Preparation of oligomer dopamine the print job. Finally, the printing was conducted using
methacrylate-poly(ethylene glycol) Phrozen DLP 3D printer, Sonic Mighty 12K (CHITUBOX,
dimethacrylate resin China), as displayed in Figure 3. The printing accuracy
Dopamine methacrylate (DMA) was initially dissolved in (% accuracy) was determined by comparing the width and
4 mL of Tris-hydrochloric acid (Tris–HCl) at a pH of 8 and length of both the large and small squares of the printed
incubated for 2 h at 37°C. This process transformed the scaffold and the designed scaffold using ImageJ software
methacrylate into ODMA, which was subsequently mixed version 1.54j.
with PEGDMA at various concentrations, i.e., 0.625–10% 2.6. Morphology analysis of scaffolds using
(w/v) ODMA. The amount of the photoinitiator was scanning electron microscopy
standardized at 0.5% (w/v) LAP in each mixture to ensure A sample of the ODMA/PEGDMA scaffold was fixed on
consistent polymerization conditions across all samples. stubs with carbon tape. To ensure the purity of the samples,
The mixture with optimal printability, containing 10% a blower is employed to remove excess powder that could
ODMA in ODMA/PEGDMA, was mixed with CH to form potentially contaminate the observations. Subsequently,
ODMA/PEGDMA/CH, which was subsequently tested the surface morphology of these scaffolds was investigated
with the chondrocyte cells. using scanning electron microscopy (SEM; FEI, USA),
2.5. Printability of resin using a digital light which was conducted at an acceleration voltage of 15 kV,
processing 3D printer utilizing magnifications of 70× and 400×.
Printability was assessed by fabricating scaffolds from 2.7. Mechanical characterization of the
ODMA/PEGDMA resin at ODMA concentrations of 10%, ODMA/PEGDMA resin
5%, 2.5%, 1.25%, and 0.625% (w/v). These scaffolds were The mechanical characterization of the ODMA–PEGDMA
designed as squares, measuring 6 × 6 mm with a thickness resin was conducted to assess the compressive modulus
of 0.8 mm. The design process involved creating a and deformation. The test specimens, in cylinders with 1.5
simulation model using SolidWorks (USA) and preparing cm diameter and 1 cm height, were fabricated from both
the slide files with CHITUBOX (China) before initiating pure PEGDMA and copolymerized with 10% (w/v) of

Volume 10 Issue 6 (2024) 341 doi: 10.36922/ijb.4385