Page 346 - IJB-10-6

International

Journal of Bioprinting

RESEARCH ARTICLE
3D-printed scaffold of dopamine methacrylate

oligomer grafted on PEGDMA incorporated
with collagen hydrolysate for engineering

cartilage tissue

Kitipong Pasanaphong 1 id , Acharee Suksuwan 2 id , Narongrit Srikaew 3 id ,
Ruedee Hemstapat 4 id , Tulyapruek Tawonsawatruk 5 id , Sutee Wangtueai 6 id ,
Nanthaphong Khamthong 7 id , Sani Boonyagul 1 id , Phavit Wongsirichot 8 id ,
and Nuttapol Tanadchangsaeng *
1 id
1 College of Biomedical Engineering, Rangsit University, Pathum Thani, Thailand
2 The Halal Science Center, Chulalongkorn University, Bangkok, Thailand
3 Research Center, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok,
Thailand
4 Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand
5 Department of Orthopaedics, Faculty of Medicine Ramathibodi Hospital, Mahidol University,
Bangkok, Thailand
6 School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, Thailand
7 College of Oriental Medicine, Rangsit University, Pathum Thani, Thailand
8 Department of Chemical Engineering, The University of Manchester, Manchester, United Kingdom

*Corresponding author:
Nuttapol Tanadchangsaeng
(nuttapol.t@rsu.ac.th) Abstract
Citation: Pasanaphong K, This study demonstrated the synthesis and characterization of dopamine
Suksuwan A, Srikaew N, et al.
3D-printed scaffold of dopamine methacrylate (DMA), oligomers of dopamine methacrylate (ODMA), and their
methacrylate oligomer grafted on integration with polyethylene glycol dimethacrylate (PEGDMA) to enhance
PEGDMA incorporated with 3D-printing scaffold fabrication for tissue engineering, using digital light processing
collagen hydrolysate for
engineering cartilage tissue. (DLP) technology. The results confirm the successful synthesis of DMA, as evidenced
Int J Bioprint. 2024;10(6):4385. by nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy
doi: 10.36922/ijb.4385 (FTIR) analysis and its subsequent conversion to ODMA. The obtained ODMA
Received: July 30, 2024 was then combined with PEGDMA (1.25–10% w/v ODMA) to optimize scaffold
Revised: August 27, 2024 printability. The morphological characteristics of the ODMA/PEGDMA scaffolds
Accepted: September 5, 2024 were assessed via scanning electron microscopy (SEM). Furthermore, using FTIR
Published Online: September 5,
2024 and differential scanning calorimetry (DSC), the chemical stability and biological
compatibility of collagen hydrolysate (CH) derived from tuna tendon were studied
Copyright: © 2024 Author(s).
This is an Open Access article and compared after sterilization. An in vitro fibroblast viability test was conducted
distributed under the terms of the using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to
Creative Commons Attribution assess the biocompatibility of CH with cells. Sterilization did not adversely affect
License, permitting distribution,
and reproduction in any medium, the chemical composition of CH, maintaining its compatibility with fibroblast cells.
provided the original work is Subsequently, ODMA/PEGDMA/CH composite scaffolds were fabricated using a DLP
properly cited. 3D printer, and their efficacy in supporting chondrocyte viability and proliferation
®
Publisher’s Note: AccScience were examined at 24, 48, and 72 h using PrestoBlue assay. Mixing ODMA with
Publishing remains neutral with PEGDMA significantly enhanced the printability of the scaffolds. Our tri-component
regard to jurisdictional claims in
published maps and institutional 3D-printed scaffolds significantly enhanced human cartilage stem/progenitor
affiliations. cell (CSPC) viability and proliferation compared to a 24-well culture plate. These

Volume 10 Issue 6 (2024) 338 doi: 10.36922/ijb.4385