Page 569 - IJB-10-6

International Journal of Bioprinting Internally-crosslinked ADA/Alg/Gel bioinks

Figure 6. In vitro characterization of ADA/Alg/Gel hydrogels (A and B) Viability (A) and toxicity (B) studies (n = 3) of ADA/Alg/Gel_50/50/0 (red), ADA/
Alg/Gel_50/40/10 (blue), and ADA/Alg/Gel_50/25/25 (green); for viability studies (A), the control group consists of AHCFs cultured on tissue culture
plastic with untreated media; for toxicity studies (B), the control group consists of AHCFs cultured on tissue culture plastic with 100% of cell death induced
by CytoTox-ONE lysis solution (grey). *p < 0.05, **p < 0.01, and ****p < 0.0001 indicate statistically significant differences between the compositions and
TM
the control group. (C and D) Live/dead assay performed on AHCF-laden ADA/Alg/Gel_50/50/0, ADA/Alg/Gel_50/40/10, and ADA/Alg/Gel_50/25/25
hydrogels at 1 h (C) and 24 h (D) after printing. Scale bars: 100 μm (C) and 500 μm (D). (E) Quantification of live (green) and dead (red) AHCFs at 24
h after printing. ****p < 0.0001 indicates a statistically significant difference between the live and dead cells for ADA/Alg/Gel_50/25/25 (n = 9). (F) Live/
dead assay performed on H9C2-laden ADA/Alg/Gel_50/25/25 hydrogels after 1 and 24 h. Scale bars: 100 μm (1h, left image) 500 μm (24h, right image).
(G) Quantification of live (green) and dead (red) H9C2 at 24 h after printing. ****p < 0.0001 indicates a statistically significant difference between the
live and dead cells for ADA/Alg/Gel_50/25/25 (n = 9). Abbreviations: AHCF: Adult human cardiac fibroblast; Alg: Alginate; ADA: Alginate dialdehyde;
Gel: Gelatin.

Volume 10 Issue 6 (2024) 561 doi: 10.36922/ijb.4014