Page 66 - IJB-7-2

3D Bioprinted Triple-layered Human Alveolar Lung Models
cultured under ALI condition expressed AT-1 biomarker models did not express the AT-1 biomarkers at day 7 as
(aquaporin-5) . However, the alveolar type II A549 lung confirmed by the aquaporin-5 and caveolin-1 staining
[72]
epithelial cells in the 3D bioprinted human alveolar lung results. There is no significant difference between the
A

B C

D E

Figure 6. (A) Confocal imaging of 3D bioprinted human blood-air barrier model at day 14 using 60× magnification. (B) Representative
image of a distinct upper layer of A549 epithelial cells – green and blue fluorescence; scale bar: 20 µm. (C) Representative image of a
distinct lower layer of EA.hy926 endothelial cells – red and blue fluorescence (nuclear DNA); scale bar: 20 µm. (D, E). Representative
composite images comprising upper layer of A549 epithelial cells and lower layer of EA.hy926 endothelial cells from top and rotated view,
respectively; scale bar: 20 µm.

62 International Journal of Bioprinting (2021)–Volume 7, Issue 2