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Jaksa, et al.
                        A                  B                  C
















           Figure 7. Printing silicone rubber on top of PLA (A), then PLA on top of silicone rubber (B), and the resulting multi-material chips from
           both tests (C) .
                        A                      B                        C















           Figure 8. Silicone-PLA multi-material ribcage model based on a medical image of a newborn during printing (A), with support after
           printing (B), and after support removal (C).
           lack of internal support, but the external geometry stayed   anatomic models in the future. The specifications stemming
           intact. This bladder was also cut in half after printing to   from this goal were printing multi-material structures
           reveal the internal cavity (Figure 6C).             out of at least one hard and one soft material, while also
               The multi-material chips from the fourth (Figure 7C)   being capable of printing empty cavities, infill structures
           and fifth (Figure 7B) tests were also printable. Moreover,   and thin-walled features. Considering the advantages and
           in  case  of  the  fifth  test,  the  PLA  top  was  deformed,   drawbacks of various AM methods and their applicable
           presumably due to printing on a soft and unstable silicone   materials, a printer was built that combines FFF and DIW
           surface. After printing, the adhesion between the silicone   technologies to print with a single-component silicone
           and the PLA in the multi-material chips was evaluated   rubber and a thermoplastic PLA filament.
           by trying to manually separate the materials. The silicone   The printing trials demonstrated that the established
           was considered adhesive enough to resist this manual   technology  is capable  of printing  objects  of both
           peeling,  since  the  bulk  silicone  material  was damaged   materials and can print silicone with a weakened internal
           before the interface.                               structure (down-tuning) or combine silicone with PLA
               After  seeing  the  success  of  the  five  previous   (up-tuning). An unsupported internal cavity and a thin-
           tests, the ribcage model of the sixth test was printed to   walled structure were also printable with the silicone. It
           demonstrate  the  applicability  of the  printer  to  produce   was shown that the FFF printhead can create hard support
           medical  image-based  anatomic  models  (Figure  8). No   structures. The strong adhesion between the PLA and the
           complications  were  experienced  during  the  printing   silicone  that  was experienced  during  the  tests  suggests
           process, although  the  manual  removal  of the  support   that this material combination can be applied to create
           structures was challenging due to the adhesion between   more  complex  multi-material  structures, and that  the
           the silicone and the PLA.                           silicone must be cut away from the PLA in case of using
                                                               PLA for printing support structures under the silicone.
           4. Discussion                                       These assumptions were confirmed through the last test,
           4.1. Overview of aims and results                   where the ribcage was indeed printable with PLA support
                                                               and sufficient adhesion between the silicone and the PLA.
           The aim of this study was to build and test a 3D printer that   These promising outcomes imply that the technology
           enables features necessary for producing more realistic   could be used to approximate the mechanical properties

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