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CNT/PCL Nanocomposite for Tissue Engineering
Figure 4. Confocal microscopy of LIVE/DEAD assay. Normal human knee articular chondrocytes (NHAC-kn) cultured for 2 weeks in
standard conditions. Scale bars represent 200 µm.
Figure 5. CellTiter-Glo 2.0 cell viability assay. Statistical analysis was performed using one-way ANOVA and post hoc Dunnett’s T3
multiple comparisons test. The mean of each column was compared to the mean of the control (Ctrl) column. CI = 95%, P value: 0.12 (ns),
0.033(*), 0.002 (**), <0.001 (***).
the 3D printing process itself resulted in a significantly hardness and elastic modulus of the PCL. The effect
increased crystallinity compared to the raw material. was the most pronounced for high concentrations of
Regarding the mechanical properties assessed by MWCNT. As mentioned previously, the main limitation
nanoindentation, the addition of CNT did increase the of the study was the inability to measure 3D-printed grids
76 International Journal of Bioprinting (2022)–Volume 8, Issue 2
