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3D Arenas for C. elegans Behavior

Appendix A

List of Parnon major parts and items used for Details on the Parnon customized print head
customization The customized print head includes the

• DOBOT MOOZ-2 machine, Shenzhen, China following parts (Fig. 2 left panel C): i) Bipolar stepper
• 12V DC Power Supply, 5V Power Supply motor of the linear actuator, to provide the actuating
• Arduino SD Cardboard, Arduino UNO force with a 40V input, controlled with Arduino. ii)
• Heating Cartridge: Insertion Heater with Internal 3D-printed custom connector (FormLabs Form2 3D
Temperature Sensor, for 3/8” Hole, 120V AC, 3” printer, clear resin v4), used to house the stroke arm
Long Heating Element, 200W, McMaster-Carr, USA (iii) and to connect the motor (i) to the heat sink
• Heating Controller: Programmable Temperature (v). iii) Stroke arm of the linear actuator with a 1.5”
Controller, for Type K Thermocouple, McMaster-Carr, total actuation range and a 1:1 stroke of 3˚μm. iv)
USA Heating element, 3” length, 3/8” diameter, 200W, set
• Limit Switch: Monoprice Maker Select 3D Printer to 65 ˚C, used to provide the temperature required to
13860 Endstop Limit Switch, Amazon, USA keep the NGM in a liquid state. v) Custom heat sink
• Linear Actuator: NEMA-8 captive, HeydonKerk, USA designed to transfer heat from the heating element
• Microstep Driver: TB6600 4A 9-42V Stepper Motor (iv) to the syringe (viii). vi) Connector (FormLabs
Driver CNC Controller, Amazon, USA Form2 3D printer, tough resin v5), used to connect
• Motor Driver: EasyDriver V4.5, SparkFun, USA the stroke arm (iii) to the plunger (vii). vii) Glass
• Peltier Devices: Northbear Thermoelectric Cooler syringe plunger which has the custom connector
Peltier Refrigeration Cooling TEC System Kits (vi) adhered on the inside. viii) Glass syringe that
Double Fan DIY +Power Supply, NorthBear, USA houses the liquid NGM. ix) Metal luer-lock nozzle.
• Thermal Compound: ARCTIC MX-4 - Thermal x) Copper wire heat induction system the prevents
Paste for Coolers, Heat Sink Paste NGM solidification in the nozzle (ix).

144 International Journal of Bioprinting (2022)–Volume 8, Issue 4