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International Journal of Bioprinting 3D bioprinting of tissue with carbon nanomaterials

Ref. [94] [95] [96] [97] [98]

Biological outcomes/ tissue engi- neering application 3D-PPF-Amine-GO and 3D-PPF- Amine-GO@BP promoted signifi- cant cell proliferation and osteogen- esis compared to 3D-PPF-Amine and 3D-PPF-Amine-BP. Bone tissue engineering ACG/GO0.1 demonstrated greater cytocompatibility and hADMSCs differentiation. Cartilage tissue engineering The 10% CNTs construct had many viable cells, whereas the more con- ductive 20% CNTs scaffold had no attached liv

MC3T3 mouse pre-osteoblast cells (30 × 10 3 cells/ well) hADMSCs (9.4 × 10 4 cells/ SH-SY5Y human neuroblas- hMSCs (0.01 × 10 6 cells/ rBMSCs (5 × 10 5 cells/ well) 8-week-old male; 3 groups, calvarial defect model of Sprague-Dawley rats

In vitro scaffold) toma cells well)

Bioprinters, crosslinking mecha- nisms, and scaffold’s dimensions PBB (VIPER si2 Stereolithography System, Valencia, CA) Photoinitiation by BAPO Orthogonal cubic lattice disks: 5 mm × 5 mm × 5 mm (L × W × H) EBB (Fourth Generation 3D Bioplotter, Envision- TEC, Germany) Ca 2+ ionic cross linkage, thermal transition, and photoinitiation by Irgacure 2959 Printed scaffold discs: 30 × 30 × 1 mm 3 (L × W × H) EBB (INKREDIBLE + Bioplotter, Cell
Table 1. List of CFNs-containing biomaterial inks for different tissue engineering applications

CFNs-containing biomaterial ink 3D-PPF-Amine, 3D-PPF-Amine- BP, 3D-PPF-Amine-GO, 3D-PPF- Amine-GO@BP ACG, ACG/GO0.1, and ACG/ GO1 NFC/alginate and NFC/alginate – 10% and 20% SWCNTs ABT, ABT-CNT3 (0.098 g), ABT-CNT7.5 (0.244 g), and ABT- CNT10 (0.325 g) PIC and PIC/MWCNTs

CFNs and dimensions GO (synthesized by the Hummers’ method; single layer sheet with a height of 1 nm) Few-layered GO (Hummers’ method; LT = 0.71 nm, LS = 1 µm) SWCNTs (Carbon Solution, River- side, CA, USA) MWCNTs (D = 10–20 nm; L = 5–50 µm) MWCNTs (D = 80 nm; L = 10 µm) (Sigma Aldrich, USA)

Volume 9 Issue 1 (2023) 187 https://doi.org/10.18063/ijb.v9i1.635