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Biocompatible materials and Multi Jet Fusion

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Figure 4. Representative scanning electron microscopy images showing the morphology of (A) L929 fibroblasts and (B) MC3T3e1 osteoblasts cells
cultured on (i) uncoated, (ii) PDL-coated, and (iii) CLG-coated 3D-printed PA-12 cell culture chambers after 4 days of culture. The red arrows are used to
specify the location of the cells. Representative scanning electron microscopy images showing the morphology of (C) L929 fibroblasts and (D) MC3T3e1
osteoblasts cells cultured on (i) uncoated, (ii) PDL-coated, and (iii) CLG-coated polystyrene cell culture chambers after 4 days of culture.
the expression pattern observed in cells cultured on shown in Figure S19. On day 28, cells cultivated in the
3D-printed PA-12 cell culture chambers was similar to differentiation medium expressed higher levels of ALP (P
that grown on the positive control (polystyrene plates) on < 0.05) than the undifferentiated one, indicating that cells
day 2 and day 4, respectively (Figure 6A). It was observed were capable of differentiation on 3D-printed PA-12. This
that only Ki67 is expressed and markedly upregulated in suggested that cells cultivated on PA-12 were capable of
cells cultured in all cell culture chambers, whereas p53 was differentiation.
downregulated (Figures S13–S18). Stronger fluorescent 3.3.7. Long-term serial passaging
signals were observed in fibroblasts in comparison to
osteoblasts. This result suggested that 3D-printed PA-12 To demonstrate the proliferative ability, cells were
cell culture chambers did not alter the gene expression of subcultured from 3D-printed PA-12 into new PA-12 cell
Ki67 and p53. culture chambers and conventional cell culture plates,
respectively, according to the experimental scheme, as
3.3.6. ALP activity shown in Figure S1. Prolonged culture of cells did not
ALP activity of differentiated MC3T3e1 osteoblasts negatively impact the cell adherence nor affected long-
cultured in 3D-printed PA-12 cell culture chambers is term serial passaging, as shown in Figure 7A.
shown in Figure 6B. The ALP activity on positive control Cells after the first and second passages also adhered and
plates (coated and uncoated) before normalization are attached to the PA-12 cell culture chambers without any sign

Volume 9 Issue 1 (2023) 24 https://doi.org/10.18063/ijb.v9i1.623