Page 118 - IJB-9-2

International Journal of Bioprinting Photocurable pullulan-based bioink for 3D printing

Figure 5. (A) Frequency dependence of the storage modulus (G′) and loss modulus (G″) of Pul-NB hydrogels with different DS. (B) Frequency-depen-
dence of G′ and G″ of Pul-NB hydrogels with different crosslinkers. (C) The viscosity of the Pul-NB precursor solution with different solid content at dif-
ferent frequency. (D) Time dependence of G′ and G″ of Pul-NB precursor solution with different solid content. (E) Frequency dependence of G′ of Pul-NB
hydrogels with different solid content. (F) Frequency dependence of G″ of Pul-NB hydrogels with different solid content.
3.3. Rheological behavior of Pul-NB precursor NB hydrogels (Pul-NB 2, Pul-NB 3, and Pul-NB 4) were
solution and hydrogels much larger than their loss modulus (G″), indicating that
The effect of DS of Pul-NB on the modulus of hydrogels the Pul-NB precursor solution formed stable hydrogels
was investigated. The Pul-NB 1 could not form hydrogel after UV curing. The G′ of Pul-NB hydrogels was
due to the excessively low DS (Figure 4A). Therefore, Pul- increased with the increase of DS, which confirmed that
NB 1 was not investigated in rheological study. It could be Pul-NB 4 had a proper DS to prepare hydrogels or 3D
seen from Figure 5A that the storage modulus (G′) of Pul- printing ink.

Volume 9 Issue 2 (2023) 110 https://doi.org/10.18063/ijb.v9i2.657