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International Journal of Bioprinting Photocurable pullulan-based bioink for 3D printing

the pressure. By employing the optimal printing process of 2. Rhee S, Puetzer JL, Mason BN, et al., 2016, 3D bioprinting
DLP technology, the Pul-NB ink could be used to print a of spatially heterogeneous collagen constructs for cartilage
series of 3D configurations with satisfactory shape fidelity. tissue engineering. ACS Biomater Sci Eng, 2(10): 1800–1805.
In summary, an optimized platform for DLP 3D printing 3. Mazzoni E, Iaquinta MR, Lanzillotti C, et al., 2021, Bioactive
technology was developed in this study. It is expected materials for soft tissue repair. Front Bioeng Biotechnol, 9: 613787.
that this novel photocurable pullulan-based ink is able to 4. Chen Z, Zhao D, Liu B, et al., 2019, 3D printing of
address the limitations of brittle and fragile photocurable multifunctional hydrogels. Adv Funct Mater, 29(20): 1900971.
hydrogel ink materials and consequently provide a possible
candidate for 3D printing of biomimetic soft tissues, soft 5. Sears NA, Wilems TS, Gold KA, et al., 2019, Hydrocolloid
robots, high-throughput tissue models, etc. inks for 3D printing of porous hydrogels. Adv Mater Technol-
US, 4(2): 1800343.
Acknowledgments 6. Zhang XN, Zheng Q, Wu ZL, 2022, Recent advances in 3D
printing of tough hydrogels: A review. Compos Part B Eng,
None. 238: 109895.

Funding 7. Qiu Z, Zheng B, Xu J, et al., 2022, 3D-printing of oxidized
starch-based hydrogels with superior hydration properties.
This work was supported by the National Key Research and Carbohydr Polym, 292: 119686.
Development Program of China [2021YFC2100900] and 8. Rajabi M, McConnell M, Cabral J, et al., 2021, Chitosan
the Tianjin Synthetic Biotechnology Innovation Capacity hydrogels in 3D printing for biomedical applications.
Improvement Project (TSBICIP-CXRC-043 and TSBICIP- Carbohydr Polym, 260: 117768.
CXRC-010).
9. Zhao D, Liu Y, Liu B, et al., 2021, 3D printing method for
tough multifunctional particle-based double-network
Conflict of interest hydrogels. ACS Appl Mater Int, 13(11): 13714–13723.
The authors declare no conflicts of interests. 10. Liu S, Bastola AK, Li L, 2017, A 3D printable and mechanically
robust hydrogel based on alginate and graphene oxide. ACS
Author contributions Appl Mater Int, 9(47): 41473–41481.

Conceptualization: Zhaoxuan Feng, Wenqin Bai, Jiaqi Lv, 11. Zielinski PS, Gudeti PKR, Rikmanspoel T, et al., 2023, 3D
Hui Song printing of bio-instructive materials: Toward directing the
Formal analysis: Zhaoxuan Feng, Jingsong Li cell. Bioact Mater, 19: 292–327.
Investigation: Zhaoxuan Feng, Jingsong Li 12. Varaprasad K, Karthikeyan C, Yallapu MM, et al., 2022,
Writing – original draft: Zhaoxuan Feng, Dasen Zhou The significance of biomacromolecule alginate for the 3D
Writing – review & editing: Zhaoxuan Feng, Wenqin Bai, printing of hydrogels for biomedical applications. Int J Biol
Jiaqi Lv, Hui Song Macromol, 212: 561–578.
13. Yuan R, Wu K, Fu Q, 2022, 3D printing of all-regenerated
Ethics approval and consent to participate cellulose material with truly 3D configuration: The critical
role of cellulose microfiber. Carbohydr Polym, 294: 119784.
Not applicable.
14. Guo J, Li Q, Zhang R, et al., 2022, Loose pre-cross-linking
Consent for publication mediating cellulose self-assembly for 3D printing strong
and tough biomimetic scaffolds. Biomacromolecules, 23(3):
Not applicable. 877–888.
15. Liu Y, Wong CW, Chang SW, et al., 2021, An injectable, self-
Availability of data healing phenol-functionalized chitosan hydrogel with fast
All data used this study are included in this published gelling property and visible light-crosslinking capability for
article and its supplementary file. Source data are provided 3D printing. Acta Biomater, 122: 211–219.
with this paper. Other relevant data are available from the 16. Abbadessa A, Blokzijl MM, Mouser VH, et al., 2016, A
corresponding author upon reasonable request. thermo-responsive and photo-polymerizable chondroitin
sulfate-based hydrogel for 3D printing applications.
References Carbohydr Polym, 149: 163–174.
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Volume 9 Issue 2 (2023) 115 https://doi.org/10.18063/ijb.v9i2.657