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International Journal of Bioprinting                          Photocurable pullulan-based bioink for 3D printing





























































            Figure 7. (A) The water absorption of Pul-NB hydrogels with different DS. (B) The water absorption of Pul-NB hydrogels with different solid content. (C)
            The lyophilized Pul-NB hydrogel absorbed water at different time points. (D) The compression stress–strain curves of Pul-NB hydrogels. (E) The com-
            pression strength of Pul-NB hydrogels. (F) The compression elastic modulus of Pul-NB hydrogels. (G) The compression stress–strain curves of Pul-NB
            and Pul-MA hydrogels with 10% solid content. (H) The procedure of compression experiments on Pul-NB and Pul-MA hydrogels. *P < 0.05; ** P < 0.01.

            OD values of all experimental groups were above 95% of   3.8. 3D printing and printing performance of
            the values of the control group, indicating that Pul-NB   Pul-NB ink
            hydrogels did not affect metabolic activity and apoptosis   The printing inks used in DLP 3D printing are not
            of L929 cells. This result preliminarily proved that Pul-NB   required to be as viscous as the inks used in 3D printing
            hydrogels had no obvious cytotoxicity. It is expected that   through extrusion technology. However, the ink must be
            this bioink may have a wide array of potential applications   transformed from a fluid liquid to solid state in a short time
            in the field of tissue engineering, drug development or   by UV irradiation. The printing of products using DLP is
            screening, toxicology screening, and tissue model of   an extremely laborious process since normal inks do not
            cancer research.                                   possess the instantaneous gel forming and self-supporting


            Volume 9 Issue 2 (2023)                        113                     https://doi.org/10.18063/ijb.v9i2.657
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