Page 184 - IJB-9-2

International Journal of Bioprinting

RESEARCH ARTICLE
Experimental study on repair of cartilage

defects in the rabbits with GelMA-MSCs scaffold
prepared by three-dimensional bioprinting

2
2
2
Zijie Pei , Mingyang Gao , Junhui Xing , Changbao Wang , Piqian Zhao ,
1,2
2
2
Hongtao Zhang *, Jing Qu *
1
1 Department of Cell Biology, School of Biology and Basic of Medical Science, Medical College of
Soochow University, Suzhou 215123, China
2 Department of Orthopedics, the First Affiliated Hospital of Soochow University, Soochow University,
Suzhou, Jiangsu, China
(This article belongs to the Special Issue: Advances in the Application of 3D Printing in Medicine
and Dentistry)

Abstract

Cartilage damage is a common orthopedic disease, which can be caused by sports injury,
obesity, joint wear, and aging, and cannot be repaired by itself. Surgical autologous
osteochondral grafting is often required in deep osteochondral lesions to avoid the
later progression of osteoarthritis. In this study, we fabricated a gelatin methacryloyl-
marrow mesenchymal stem cells (GelMA-MSCs) scaffold by three-dimensional (3D)
*Corresponding authors bioprinting. This bioink is capable of fast gel photocuring and spontaneous covalent
Hongtao Zhang cross-linking, which can maintain high viability of MSCs and provide a benign
(htzhangsz@163.com)
Jing Qu microenvironment to promote the interaction, migration, and proliferation of cells.
(qujing@suda.edu.cn) In vivo experiments, further, proved that the 3D bioprinting scaffold can promote the
regeneration of cartilage collagen fibers and have a remarkable effect on cartilage
Citation: Pei Z, Gao M, Xing J,
et al., 2023, Experimental study repair of rabbit cartilage injury model, which may represent a general and versatile
on repair of cartilage defects in the strategy for precise engineering of cartilage regeneration system.
rabbits with GelMA-MSCs scaffold
prepared by three-dimensional
bioprinting. Int J Bioprint, Keywords: Three-dimensional bioprinting; Mesenchymal stem cells; Gelatin
9(2): 662.
https://doi.org/10.18063/ijb.v9i2.662 methacryloyl-marrow mesenchymal stem cells scaffold; Cartilage regeneration;
MicroRNA-410
Received: July 13, 2022
Accepted: October 11, 2022
Published Online: January 5, 2023 1. Introduction
Copyright: © 2023 Author(s).
This is an Open Access article Osteoarthritis (OA) is a common degenerative joint disease. A considerable part of
distributed under the terms of the OA is caused by the degeneration of articular cartilage, which is the elastic connective
Creative Commons Attribution [1]
License, permitting distribution, tissue within the joint . Normal articular cartilage, also known as hyaline cartilage,
and reproduction in any medium, is composed of chondrocytes, matrix, and collagen fibers. The extracellular matrix
provided the original work is (cartilage matrix) in cartilage is rich in type II collagen and proteoglycan, forming a
properly cited. dense network in physiological cartilage that generates sufficient mechanical strength .
[2]
Publisher’s Note: Whioce Osteochondral defects are common in traumatic injury or disease, and the injured joint
Publishing remains neutral with space lacks cartilage-secreted synovial fluid, resulting in pain during active contact .
[3]
regard to jurisdictional claims in
published maps and institutional However, due to the lack of vascular, nerve, and lymphatic networks, pathological
affiliations. changes involving cartilage often lead to irreversible full-thickness osteochondral

Volume 9 Issue 2 (2023) 176 https://doi.org/10.18063/ijb.v9i2.662