Page 127 - IJB-9-3

International Journal of Bioprinting

RESEARCH ARTICLE
Programmable and printable formaldehyde

dehydrogenase as an excellent catalyst for
biodegradation of formaldehyde

Shuyan Zhao , Bo Zeng , Rui Pei , Xiaoli Fu , Meinan Zhu , Guangya Zhang ,
1
2
1
1
1
1
Wei Jiang *
1
1 College of Chemical Engineering, Huaqiao University, Xiamen, Fujian, 361021, China
2 School of Environmental Science and Engineering, Shandong University, Qingdao 266237, China
Abstract

As an environmental pollutant, formaldehyde can cause serious harm to the human
body. Among many degradation methods, formaldehyde dehydrogenase from
Pseudomonas putida (PFDH) exhibits broad potential because of its strong catalytic
specificity and high degradation efficiency. However, the real application of PFDH
in industry is limited by its instability and difficulties in recycling. In this work, the
suitable printing conditions for immobilizing PFDH by three-dimensional (3D)
printing technology were studied: the concentration of sodium alginate (SA) was
1.635 wt%, the concentration of CaCl was 7.4 wt%, the crosslinking time with CaCl 2
2
was 8 min, and the temperature of the reaction was 31.5°C. 3D-printed PFDH/calcium
alginate (CA) microspheres have 210% relative enzyme activity after seven repeated
uses. Dried PFDH/CA particles were characterized by scanning electron microscope
*Corresponding author: (SEM), Fourier transform infrared spectrometer (FT-IR), EDS elemental mapping, and
Wei Jiang (wjiang@hqu.edu.cn)
thermogravimetric analysis (TGA) which proved that the enzyme was immobilized by
Citation: P Zhao S, Zeng B, the material. In addition, the recycling ability of 3D printing to immobilize different
Pei R, et al., 2023, Programmable objects was explored and different shapes were designed by computer-aided design
and printable formaldehyde
dehydrogenase as an excellent (CAD). In conclusion, 3D printing technology was applied to immobilize PFDH in this
catalyst for biodegradation of work, which provides a new idea to biodegrade formaldehyde in a green way.
formaldehyde. Int J Bioprint,
9(3): 695.
https://doi.org/10.18063/ijb.695
Keywords: Formaldehyde dehydrogenase; Enzyme immobilization; 3D printing;
Received: September 14, 2022 Degradation of formaldehyde
Accepted: November 06, 2022
Published Online: February 27,
2023
Copyright: © 2023 Author(s). 1. Introduction
This is an Open Access article
distributed under the terms of the Biocatalysis of enzymes has the potential to produce high value-added chemicals, which
Creative Commons Attribution are playing an increasingly important role in our life . Formaldehyde is an organic
[1]
License, permitting distribution
and reproduction in any medium, substance that reacts nonspecifically with proteins, nucleic acids, and lipids. It is highly
provided the original work is toxic to living organisms as it can cause death or mutation. Formaldehyde is listed as the
properly cited. first group of major carcinogenic organic compounds in the world by the International
[2]
Publisher’s Note: Whioce Agency on Cancer (IARC) . Formaldehyde is widely used in our daily lives; for
Publishing remains neutral with example, they are used in interior decorations such as paints, carpets, and wallpaper,
regard to jurisdictional claims in
published maps and institutional and in everyday products such as disinfectants and antiseptics, as well as released in
[3]
affiliations. industrial waste water or gases . Formaldehyde is an environmental pollutant [4,5] .

Volume 9 Issue 3 (2023) 119 https://doi.org/10.18063/ijb.695