Page 132 - IJB-9-3

International Journal of Bioprinting Programmable formaldehyde dehydrogenase for biodegradation formaldehyde

Figure 2. The effect of different immobilized conditions on activity of PFDH was analyzed by RSM. (A) SA concentration and cross-linking. (B) SA
concentration and temperature. (C) SA and CaCl concentration. (D) Crosslinking time and CaCl concentration. (E) Temperature and CaCl concentration.
2
2
2
(F) Crosslinking time and temperature.
different. Therefore, we need to investigate the properties
of immobilized PFDH/CA microspheres.

3.4. Recycling ability of immobilized PFDH/CA
microspheres
To obtain the best size of the particle, we studied the
recycling ability of PFDH/CA microspheres with the same
quality. The recycling performance of immobilized PFDH/
CA microspheres of different sizes but with the same mass
was measured. The maximum enzyme activity at the time
of the first test was defined as 100% (Figure 4). The relative
enzyme activity of 1.5 mm PFDH/CA microspheres was
maintained at 98% after five repeated uses; the relative
enzyme activity of 2.5 mm PFDH/CA microspheres was
maintained at 210% after seven repeated uses; the relative
enzyme activity of 3.5 mm PFDH/CA microspheres
was maintained at 184% after reuse for six times. Three
microspheres with different diameters had similar
Figure 3. PFDH/CA microspheres immobilized by 3D printing technology. recycling trends, but microspheres with the diameter of

Volume 9 Issue 3 (2023) 124 https://doi.org/10.18063/ijb.695