Page 136 - IJB-9-3

International Journal of Bioprinting Programmable formaldehyde dehydrogenase for biodegradation formaldehyde

Figure 7. Degradation of formaldehyde by the immobilized PFDH/CA microspheres over time.

Figure 8. The reuse of immobilized different particle objects. (A) Pure enzyme, (B) crude enzyme, and (C) recombinant E. coli.
printing can be used to print different shapes with the reuse performance. The 2.5 mm immobilized PFDH/CA
use of computer [1,46,47] . This technique can be used to microspheres still have a relative activity of 210% after
remove formaldehyde in printed objects, print objects in seven repeated uses. 3D printing of different objects (pure
controllable shapes so as to adapt to the different industrial enzyme, crude enzyme, recombinant E. coli) or shapes
requirements, and produce objects for interior decoration, was explored with the aim of translating the technique
as shown in Figure 9. into industrial applications. Besides, direct printing of
recombinant E. coli also has good reusability properties.
All in all, this study provides a green method for the
4. Conclusion degradation of formaldehyde.
In summary, we successfully immobilized PFDH using
3D printing technology and demonstrated its utilization Acknowledgments
in the biodegradation of formaldehyde. Based on single-
factor experiments, RSM-optimized conditions for None.
3D printing of PFDH are as follows: 1.635 wt% for SA,
7.4 wt% for CaCl , 8 min for CaCl crosslinking, and Funding
2
2
31.5°C for the reaction. PFDH was immobilized by 3D The work was supported by the National Natural Science
printing to remove formaldehyde, showing excellent Foundation of China (No.21808073), Natural Science

Volume 9 Issue 3 (2023) 128 https://doi.org/10.18063/ijb.695