Page 140 - IJB-9-3

International Journal of Bioprinting

RESEARCH ARTICLE
Co-culture bioprinting of tissue-engineered

bone-periosteum biphasic complex for repairing
critical-sized skull defects in rabbits

Danyang Zhao , Yu Wang , Zhencheng Yu , Chuandong Wang ,
1†
3
1
2†
Hongbo Zhang *, Dong Han *, Qingfeng Li *
4
1
1
1 Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai
Jiao Tong University School of Medicine, Shanghai, China
2 Department of Cardiology, Shidong Hospital, Yangpu District, Shidong Hospital Affiliated to
University of Shanghai for Science and Technology, Shanghai, China
3
Department of Orthopedic Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University
School of Medicine, Shanghai, China
4 School of Mechanical and Power Engineering, East China University of Science and Technology,
Shanghai, China
(This article belongs to the Special Issue: Novel Methods, Processes, and Materials of Bioprinting)
† These authors contributed equally
to this work. Abstract
*Corresponding author:
Qingfeng Li Tissue engineering based on bioprinting technology has broad prospects in the
(dr.liqingfeng@shsmu.edu.cn) treatment of critical-sized bone defect. Nevertheless, it is challenging to construct
Dong Han
(handong12000@163.com) composite tissues or organs with structural integrity. Periosteum and stem cells are
Hongbo Zhang important in bone regeneration, and it has been shown that co-culture engineering
(hbzhang@ecust.edu.cn) system could successfully repair bone defects. Here, a strategy of co-culture
Citation: Zhao D, Wang Y, Yu Z, bioprinting was proposed, and a tissue-engineered bone-periosteum biphasic
et al., 2023, Co-culture bioprinting of complex was designed. Poly-L-lactic acid/hydroxyapatite (PLLA/HA) was used to
tissue-engineered bone-periosteum construct the supporting scaffold of bone phase. Gelatin methacryl (GelMA) loaded
biphasic complex for repairing
critical-sized skull defects in rabbits. with rabbit bone mesenchymal stem cells (BMSCs) and periosteum-derived stem
Int J Bioprint, 9(3): 698. cells (PDSCs) were used to simulate the extracellular matrix and cellular components
https://doi.org/10.18063/ijb.698 of bone and periosteum, respectively, and a co-culture layer was formed between
Received: August 02, 2022 the bone and the periosteum phase. By adjusting material ratios of PLLA/HA and
Accepted: October 08, 2022 crosslinking time of GelMA, a complex with good mechanical strength and cell
Published Online: March 2, 2023
activity was constructed and then implanted into the defect area of rabbit skull.
Copyright: © 2023 Author(s). The quantitative results of imaging and histology showed that the repair effect of
This is an Open Access article
distributed under the terms of the bone-periosteum biphasic complex group was significantly better than that of other
Creative Commons Attribution control groups, which demonstrated that the bone-periosteum biphasic complex
License, permitting distribution was advantageous to both bone repair and regeneration. In general, using the
and reproduction in any medium,
provided the original work is co-culture bioprinting to construct engineered tissue is a very promising strategy,
properly cited. which is expected to be applied in the construction of more complex tissues and
solid organs for tissue repair and organ transplantation.
Publisher’s Note: Whioce
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: Bioprinting; Complex; Co-culture; Stem cells; Bone defect
affiliations.

Volume 9 Issue 3 (2023) 132 https://doi.org/10.18063/ijb.698