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International Journal of Bioprinting                                             3D-Printed liver model


























            Figure 3. Tensile testing specimens after slicing in Prusa Slicer, with a liver-matching specimen (bottom) and a full-silicone benchmark specimen (top)
            displayed with the top layers hidden, to compare the inside of the specimens.























                                    Figure 4. (A) Printing the liver model. (B) Printing a tensile testing specimen.
            is the initial cross-sectional area, measured with a caliper   energy is referred to as the dissipated energy ratio. Besides
            before testing.                                    plotting the stress–strain curves of all four loading-
                                                               unloading cycles and the initial and final elastic moduli
               Meanwhile, the uniaxial linear engineering stress (σ)
            was calculated using Equation II:                  on the last loading cycle, the dissipated energy ratios for
                                                               the second, third, and fourth cycles were also calculated.
                   f                                           In this regard, the first loading cycle was ignored due to
                                                     (II)
                   A 0                                         distortions coming from the preload.
            where f is the measured axial force and A  is the initial   2.6. Imaging
                                               0
            cross-sectional area.                              Due to the lack of respective data on fluid-filled structures
               The ratio of dissipated energy over a loading-unloading   and the fact that the chosen design logic of the liver model
            cycle was taken a measure of the overall viscous behavior of   did not allow for deliberate tuning for radiological properties,
            the multi-material structure. The total energy of a loading   achieving a degree of imaging realism was only an option
            cycle is the area under the stress–strain curve of the loading   but nevertheless a preferred outcome. Therefore, the liver
            half-cycle, while the dissipated energy of a loading cycle is   model underwent CT scanning (SOMATOM Definition AS,
            the area within the hysteresis loop formed by the stress–  Siemens Healthineers, Erlangen Germany) with a modified
            strain curves of loading and unloading half-cycles . The   clinical CT protocol to evaluate the radiodensity properties.
                                                    [48]
            dimensionless ratio of the dissipated energy to the total   The CT scan settings are listed in Table 3.

            Volume 9 Issue 4 (2023)                         95                         https://doi.org/10.18063/ijb.721
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