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International Journal of Bioprinting Blood components for tissue graft bioprinting

Evaluation of the output/biological aspects. Proliferation,

rabbit osteochondral lesion. migration and differentiation. In vivo M2 infiltration in a Mechanical and degradation tests/rheology Cell proliferation, viability, histology, immunohistochemical analyses, qRT-PCR, COL-II, COL-I, COL-X, ACAN SEM, swelling assay, rheology, Fourier transform infrared spectroscopy (FTIR), biocompatibility Cardiac cell-laden constructs To validate the applicability of this scaffold toward mimicking cardiac tissue Tissue engineered vascu

Blood-derived product PRP double-spinning Plt 8× (from New Zealand rabbits) TGF-β1, PDGF-AB Fibrinogen Thrombin (#) Fibrinogen (#) Fibrinogen (#) Fibrin (#) PRP hPL, 3 batches from pooled donors (blood bank) hPL, fibrinogen Fibrinogen from bovine plasma and thrombin (#) FFP (30 mg fibrinogen/13 mL FFP) Fibrinogen (from bovine plasma) Thrombin (#) PRP PPP PRP PPP PRP (scaffold coating) Fibrinogen (from bovine plasma) Thrombin (#) PRP,

Modality/cell phenotype. Printing/BMSC Rabbit chondrocytes Human-induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CM) Human CM AC16 cell lines (ATCC) Rotary bioprinting / neonatal human dermal fibroblasts HUVEC iPSC-CM coaxial needle system HUVEC H9c2 Corneal epithelial cells No cells Human fibroblasts Human keratinocytes Human fibroblasts mixed with ink Keratinocytes seeded on top UC-MSCs Human dermal fibroblasts

Ink formulation. GelMA/PRP Two components mixed 1:1: 80% PEG • 10% Silk fibroin dissolved in 12.5%, 25% or • 50% PRP Crosslinking with PEG 400 1 mL of the bioink: 990 μL of fibrinogen solution (60 mg/mL), 155 mg of gelatin- furfuryl at 25°C Heat it at 37 °C for 1 h with stirring, add Rose Bengal (10 μL) Polymerization: 50 units/mL thrombin, and 80 mM CaCl 2 Gelatin Fibrinogen crosslinked with thrombin Alginate, PEG Alginate and polyethylene glycol monoa

Author (year); Journal Li et al. (2020); Tissue Engineering [53] Kumar et al. (2019) ; ACS Biomater Sci Eng [54] Cardiac and vascular grafts Freeman et al. (2019); Acta Biomater [55] Maiullari et al. (2018); Scientific Reports [56] Wang et al. (2018); Acta Biomater [57] Zou et al. (2020); Mater Sci Engineering C [46] Corneal ulcers Frazer et al. (2020); Trans Vis Sci Tech [58] You et al. (2022); Trans Vis Sci Tech [59] Dermal patch

Volume 9 Issue 5 (2023) 285 https://doi.org/10.18063/ijb.762