Page 291 - IJB-9-5

International Journal of Bioprinting Blood components for tissue graft bioprinting

Evaluation of the output/biological aspects. Proliferation,

rabbit osteochondral lesion. migration and differentiation. In vivo M2 infiltration in a Proliferation Migration Differentiation Bone rigidity In vitro: Mechanical properties, cell proliferation-osteogenic differentiation, macrophage polarization. In vivo: Subcutaneous implantation of bone grafts to assess vascularization, enhanced vascularization with PRP- GA&laponite/PCL; implantation of bone grafts in rat femoral condyle defects, X-ray and

Table 1. 3D bioprinting of specific tissue constructs using bioinks functionalized with blood-derived biomaterials

Blood-derived product FFP Rat PRP Autologous PRP (4 mL) PRP, prepared “in house,” with high platelet count and high concentration of VEGF, PDGF-AA, bFGF, TGF-β3 Fibrinogen Thrombin (#) PRP PRP

Modality/cell phenotype. Printing/BMSC Bioprinting/ MSCs Bioprinting/ rat BMSC No cells 3D printing / hASCs seeded post-printing Printing bioprinting / MSCs spheroids printing/BMSC Bioprinting/ATDC5 murine chondrocyte cell line

Ink formulation. GelMA/PRP 3 w/v% alginate and 9 w/v% methylcellulose, a pasty bioink (plasma-Alg-MC) CPC (calcium phosphate cement) Three bioinks: GA/PCL, PRP-GA/PCL, and PRP-GA@laponite/PCL GA composite hydrogel: GelMA/AlgMA (5:1) PCL/β-TCP/PRP Silk fibroin, HA, gelatin, TCP 3D scaffold printing coated with PRP Sterilization for 2 h using UV and 70% ethanol PEG 20%–alginate 2.5% hydrogel containing 0.25% photoinitiator and supplemented with 1

Author (year); Journal Bone Ahlfeld et al. (2020); ACS Applied Materials & Interfaces [47] Cao et al. (2023); Int J Bioprint [48] Hao et al. (2023) ; Int J Bioprint [49] Wei et al. (2019); Bioactive Materials [50] Cartilage de Melo et al. (2019); Adv Funct Mater [43] Jiang et al. (2021); Acta Biomater [51] Irmak and Gümüşderelioglu (2020); Biomedical Materials [52]

Volume 9 Issue 5 (2023) 283 https://doi.org/10.18063/ijb.762