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International Journal of Bioprinting Blood components for tissue graft bioprinting

Evaluation of the output/biological aspects. Proliferation,

rabbit osteochondral lesion. migration and differentiation. In vivo M2 infiltration in a Proliferation of Schwann cells. Peripheral nerve repair, fabrication of functional nerve conduits, bridging a 10-mm gap in sciatic nerve in rats Reduction of muscle fiber atrophy. No changes in re-myelination and neural protein expression Biofabrication of dentin pulp complexes In vitro: Viability of cell proliferation, gene expression of COL1 and COL-III

Blood-derived product Platelets (fluorescent marker) Encapsulated intact platelets within hydrogel Fibrin (#) Anti-coagulated blood to prepare PRF single spinning Alginate was dissolved in either PL (n = 20 donors) FFP (n = 5 donors) PRP reaction; SE, standard error; SEM, scanning electron microscopy; TCP, tricalcium phosphate; TGF-β, transforming growth factor beta; UC-MSCs, mesenchymal stem cells from umbilical cords; UV,

Modality/cell phenotype. Printing/BMSC Schwan cells HUVEC DLP printer Dental pulp stem cells Human gingival fibroblasts Release kinetics of PDGF-AB, TGF-β1, VEGF, EGF, FGF, and PDGF-AA Pancreas neonatal porcine islet-like cell clusters macroencapsulation Islet organoid Table 1 shows the state of the art of extrusion bioprinting using bioinks doped with blood-derived product. The constructs aim to mimic both hard/semi-hard and so

Ink formulation. GelMA/PRP PEGDA, GelMA Fibrin Alginate 4%/gelatin 8% 3% alginate (dissolved in bovine serum albumin or platelet lysate (PL) or FFP)+ 9% MC PBS-algMC, BSA-algMC, PL-algMC, FFP- algMC GelMA ECM (#) indicates commercially available lyophilized fibrin.

Table 1. Continued Author (year); Journal Tao et al. (2020); Adv Funct Mater [68] Oral tissues Han et al. (2019); J Tissue Engineering [69] Yi et al. (2022); Regenerative Biomaterials [70] Pancreas Duin et al. (2022); Biomedicines [71] Zhu et al. (2023); Colloids and Surfaces B: Biointerfaces [45] added later to the bioprinted construct. ultraviolet; VEGF, vascular endothelial growth factor.

Volume 9 Issue 5 (2023) 286 https://doi.org/10.18063/ijb.762