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International Journal of Bioprinting 3D-printed Mg scaffolds promote bone defect repair

Figure 3. Cytocompatibility and osteogenic differentiation of rBMSCs cultured in the extract of naked JDBM (Mg), Mg/Sc, and Mg/Sc/ZA samples,
respectively. (A) Cell viability. (B) ALP staining after 7 days of culture. (C) Alizarin red staining at 21 days. (D) Statistical analysis of relative activity
of ALP at 7 days. (E) Semi-quantitative analysis of alizarin red staining for 21 days. (F) Relative expression of osteogenic differentiation related genes.
*P < 0.05 vs. Control group; P < 0.05 vs. Mg group.
#
severely damaged, and there were many degradation slow and controllable, and the release rate was slow at first,
products on the surface of the scaffolds. Corrosion of the then fast, and finally decreased until it gradually stabilized.
scaffolds was the least in the Mg/Sc/ZA group, and there The release rate of ZA was similar to that of the scaffolds,
was almost no corrosion cracking on the scaffolds. During indicating that the release of ZA was mainly controlled by
2+
the entire in vitro degradation process, the pH, Mg degradation of the drug-loaded coating. The proportion of
concentration, and hydrogen release of the Mg group were ZA released in the first 3 days was approximately 8%, and
significantly higher than those of the Mg/Sc and Mg/Sc/ the cumulative release rate of ZA was 54% after soaking
ZA groups (Figure 2B–D). Additionally, the degradation for 30 days.
rates of the Mg/Sc and Mg/Sc/ZA groups were significantly 3.3. In vitro cytocompatibility
lower than those of the uncoated Mg alloy scaffolds in the The effect of the 3D-printed Mg alloy scaffold extracts
Mg group (Figure 2E).
from different groups on the proliferation of BMSCs
The in vitro drug release results are shown in Figure 2F. from osteoporotic rats in vitro is shown in Figure 3A. Cell
The release of ZA from the scaffold coating in vitro was proliferation in the Mg/Sc and Mg/Sc/ZA groups was

Volume 9 Issue 5 (2023) 408 https://doi.org/10.18063/ijb.769