Page 50 - IJB-9-5

International Journal of Bioprinting Precise fabrication of engineered vascular networks

Figure 3. Effect of P/G concentration on swelling compensation. (A) Images of hydrogel scaffolds with vasculature prepared with different P/G
concentrations at various shrinking time points. (B) Diameter of printed sacrificial PF-127 fibers and vasculature at different time points. (C) Shrinkage
ratio of hydrogel scaffolds with vasculature prepared with different P/G concentrations. n = 3 for each group.

3.3. Effect of vasculature density on the swelling of sacrificial PF-127. After complete crosslinking, the
compensation sacrificial template was removed, and the P/G hydrogel
The vasculature increases the surface area for the P/G scaffold with the 35-mm Petri dish was floated in the water
hydrogel scaffold with the air, which provides a channel for bath at 37°C to observe the shrinking process. Red acrylic
the water to be released from the hydrogel when placed paint was injected into the vasculature to distinguish
at 37°C. Thus, the effect of vasculature density on the various densities.
swelling compensation was explored in this section. Three
patterns with three, six, and nine fibers of vasculature in The images of P/G hydrogel scaffolds during the
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Figure 4A were designed. P/G , P/G , and P/G were used shrinking process were captured, as shown in Figure 4B.
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in this section. A 20-G needle was used for the printing Images of P/G and P/G hydrogel scaffolds are displayed
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Volume 9 Issue 5 (2023) 42 https://doi.org/10.18063/ijb.749