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International Journal of Bioprinting Biofabrication for islet transplantation

Figure 2. Application of natural hydrogels. (A) Schematic image of the encapsulation of islets using a balanced charged hydrogel that results in
immunoprotection and controlled insulin secretion. (B) Representative histology and immunofluorescence images of retrieved hydrogel samples from
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Alginate/PEI and alginate-treated groups at 90 days post-transplantation. Adapted with permission from reference . Copyright © 2019 WILEY‐VCH
Verlag GmbH & Co. KGaA, Weinheim. (C) Schematic image and gross view of the 3D reconstruction of SC β cells in the DP. (D) Immunofluorescence
staining images of cell discs in DP and gene expression analysis showing higher expression of E-cadherin in SC β cells cultured in DP compared to 2D
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culture conditions. Adapted with permission from reference . Copyright © 2020 AAAS. (E) Schematic image of the fabrication process of a silk-based
bioartificial pancreas. (F) Stimulation index of primary islets encapsulated in different scaffolds and immunofluorescence images of primary islets cultured
in different scaffolds after 14 days. (G) Gene expression analysis for M1 and M2 macrophage surface markers when incubated with IL-4-loaded silk-alginate
scaffolds for 72 h. Comparative analysis of tumor necrosis factor-alpha produced by macrophages, suggesting lower levels of inflammatory responses in
scaffolds. H&E staining and immunofluorescence staining images of retrieved acellular scaffolds after 4 weeks of subcutaneous implantation in mice.
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Adapted with permission from reference . Copyright © 2017 American Chemical Society.

Volume 9 Issue 6 (2023) 396 https://doi.org/10.36922/ijb.1024