Page 107 - v11i4

International

Journal of Bioprinting

REVIEW ARTICLE
Three-dimensional bioprinting in human-

relevant toxicology: Advanced organ models and
translational strategies

Yinpeng Le , Tanqing Long , Qi Wang , Mengcheng Tang ,Mingyue Pan ,
2
1,2
2,3
1
2,4
Qingru Song , Wenrui Ma , Yuxin Su , Yutian Feng , Ni An , Wenzhen Yin ,
5
2,5
5
2
5
2
* , and Juan Liu *
Xiangdong Kong * , Yunfang Wang 2,5,6 id 2,6 id
1 id
1 Zhejiang-Mauritius Joint Research Center for Biomaterials and Tissue Engineering, School of
Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China
2 Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, School of Clinical
Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China
3
Department of Pharmacology, School of Pharmacy, Tongji Medical College, Huazhong University
of Science and Technology, Wuhan, Hubei, China
4 State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Drug Discovery for
Metabolic Diseases, Center of Advanced Pharmaceuticals and Biomaterials, China Pharmaceutical
University, Nanjing, Jiangsu, China
*Corresponding authors:
Juan Liu 5 Clinical Translational Science Center, Beijing Tsinghua Changgung Hospital, Tsinghua Medicine,
(lja02720@btch.edu.cn) Tsinghua University, Beijing, China
Yunfang Wang 6 Ministry of Education Key Laboratory of Digital Intelligence Hepatology, School of Clinical
(wangyf008@mail.tsinghua.edu.cn) Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China
Xiangdong Kong
(kongxd@zstu.edu.cn)
Citation: Le Y, Long T, Wang Qi,
et al. Three-dimensional bioprinting Abstract
for human-relevant toxicology:
Advanced organ models and Traditional toxicological testing, which relies on animal models and two-dimensional
translational strategies.
Int J Bioprint. 2025;11(4):99-132. cell cultures, encounters challenges in accurately predicting human-specific
doi: 10.36922/IJB025210209 responses due to interspecies variability and the inherent limitations of simplified
in vitro systems. Three-dimensional (3D) bioprinting has emerged as a transformative
Received: May 21, 2025
Revised: June 25, 2025 approach, facilitating the fabrication of physiologically relevant tissue constructs with
Accepted: July 3, 2025 precise spatial control over cellular and extracellular matrix components. This review
Published online: July 3, 2025 critically examines recent advancements in 3D-bioprinted organ models, such as the
Copyright: © 2025 Author(s). liver, kidney, and lung, for toxicological assessments, including their applications in
This is an Open Access article drug safety evaluation, environmental pollutant screening, and nanomaterial risk
distributed under the terms of the
Creative Commons Attribution assessment. We further analyze persistent technical barriers concerning resolution
License, permitting distribution, limitations, material biocompatibility, and the simulation of multi-organ interactions.
and reproduction in any medium, Finally, we propose integrative strategies that combine organ-on-a-chip platforms,
provided the original work is
properly cited. artificial intelligence-driven design, and standardized validation protocols, aiming to
accelerate the translational potential of bioprinted models in regulatory toxicology.
Publisher’s Note: AccScience
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: Functional simulation; Organ models; Three-dimensional bioprinting;
affiliations. Toxicology testing

Volume 11 Issue 4 (2025) 99 doi: 10.36922/IJB025210209