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International Journal of Bioprinting                               3D-printed microstructure for bacteriostasis




            Natural Science Foundation of China (61904057), Natural      doi: 10.1128/mmbr.00014-09
            Science Foundation of Hubei Province (2018CFB124),   5.   Akcalı A, Lang NP. Dental calculus: the calcified biofilm
            and Research Programs of Science and Technology,      and its  role  in disease development.  Periodontol 2000.
            Department of Education of Hubei Province (B2023256)   2018;76(1):109-115.
            to B. Cai, and International Orthodontics Foundation      doi: 10.1111/prd.12151
            Young Research Grants Award (IOF2024Y11) to F.-Y. Huo.
                                                               6.   Karygianni L, Ren Z, Koo H, Thurnheer T. Biofilm
                                                                  matrixome: extracellular components in structured microbial
            Conflict of interest                                  communities. Trends Microbiol. 2020;28(8):668-681.
            The authors declare they have no competing interests.     doi: 10.1016/j.tim.2020.03.016
                                                               7.   Arciola CR, Campoccia D, Montanaro L. Implant infections:
            Author contributions                                  adhesion, biofilm formation and immune evasion. Nat Rev
                                                                  Microbiol. 2018;16(7):397-409.
            Conceptualization: Fang-Yi Huo, Wentao Zhu, Kan Zhou,      doi: 10.1038/s41579-018-0019-y
               Enduo Zhou, Lei-Ming Cao, Qian Zhu, Hong He
            Funding acquisition: Hong He                       8.   Sharma D, Misba L, Khan AU. Antibiotics versus biofilm:
            Investigation: Fang-Yi Huo, Wentao Zhu, Kan Zhou      an emerging battleground in microbial communities.
            Methodology: Fang-Yi Huo, Wentao Zhu, Kan Zhou,       Antimicrob Resist Infect Control. 2019;8:76.
                                                                  doi: 10.1186/s13756-019-0533-3
               Enduo Zhou, Lei-Ming Cao, Qian Zhu
            Project administration: Bo Cai, Lin-Lin Bu, Hong He  9.   Roy R, Tiwari M, Donelli G, Tiwari V. Strategies for
            Supervision: Bo Cai, Lin-Lin Bu, Hong He              combating bacterial biofilms: a focus on anti-biofilm
            Visualization: Fang-Yi Huo                            agents and their mechanisms of action.  Virulence. 2018;
            Writing–original draft: Fang-Yi Huo, Wentao Zhu,      9(1):522-554.
               Kan Zhou, Enduo Zhou, Lei-Ming Cao, Qian Zhu       doi: 10.1080/21505594.2017.1313372
            Writing–review & editing: All authors              10.  Wu H, Moser C, Wang HZ, Høiby N, Song ZJ. Strategies
            All authors have reviewed and approved the final version   for combating bacterial biofilm infections.  Int J  Oral Sci.
               of this manuscript for publication.                2015;7(1):1-7.
                                                                  doi: 10.1038/ijos.2014.65
            Ethics approval and consent to participate         11.  Nwabuife JC, Omolo CA, Govender T. Nano delivery
            Not applicable.                                       systems to the rescue of ciprofloxacin against resistant
                                                                  bacteria “E. coli; P. aeruginosa; Saureus; and MRSA” and
                                                                  their infections. J Control Release. 2022;349:338-353.
            Consent for publication                               doi: 10.1016/j.jconrel.2022.07.003
            Not applicable.                                    12.  Fu X, Rehman U, Wei L, et al. Silver-dendrimer
                                                                  nanocomposite as emerging therapeutics in anti-bacteria
            Availability of data                                  and beyond. Drug Resist Updat. 2023;68:100935.
            Data  are  available  from  the  corresponding  author  upon      doi: 10.1016/j.drup.2023.100935
            reasonable request.                                13.  Kalghatgi S, Spina CS, Costello JC, et al. Bactericidal
                                                                  antibiotics induce mitochondrial dysfunction and
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            Volume X Issue X (2025)                        162                            doi: 10.36922/IJB025150135
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